AmplifyRP® XRT for RsR3B2 (Ralstonia solanacearum Race 3, Biovar 2)
AmplifyRP® XRT for RsR3B2 is a rapid DNA amplification and detection platform designed for field-based or laboratory testing of Ralstonia solanacearum Race 3, Biovar 2 in many crops including: banana, eggplant, geranium, ginger, pepper, potato, and tomato. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify RsR3B2 DNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
If you would prefer to add a field-deployable serological assay to your testing toolkit, please check out Agdia's Rs ImmunoStrips® (Will detect all Ralstonia solanacearum species complex samples).
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT
|Limit of detection: 100 fg/µL purified DNA, ~5x103 CFU/mL|
|Isolate Names / Geographic Strain Locations|
|RsR3B2-CFBP8695 (Iran1)||RsR3B2-IPO2609 (Philippines1)|
|RsR3B2-MI20 (MI, USA1)||RsR3B2-PS-11 (Guatemala1)|
|RsR3B2-UW19 (Columbia1)||RsR3B2-UW276 (Mexico1)|
|RsR3B2-UW365 (China1)||RsR3B2-UW551 (Kenya1)|
|1Location is geographical origin, not presence location.|
For a list of validated hosts, please see the Full Validation Report.