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AmplifyRP® XRT for Fo (Fusarium oxysporum)

Image of Amplifire isothermal fluoromter showing amplification Click to view a larger image
Part Number:
XCS 70500/0048
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AmplifyRP® XRT for Fo is a rapid DNA amplification and detection platform designed for testing cannabis plants for Fusarium oxysporum. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Fo DNA and an endogenous DNA control at a single operating temperature (42 °C).

AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.

The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.

Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.

Click here for other product documents such as the User Guide or printable barcode.

Included:

  • XRT reaction pellets for Fo
  • Pre-filled 100 µL PD1 Pellet diluent tubes
  • GEB-filled mesh extraction bags
  • User Guide

Required but Not Included:

Optional:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: ROX-labeled control probe (endogenous)

Full Validation Report

Analytical Sensitivity
Limit of detection: Approximately 32 - 72 copies of DNA fragments

Formae Speciales Detected
Fusarium oxysporum f.sp. albedinis1 Fusarium oxysporum f.sp. asparagi1
Fusarium oxysporum f.sp. batatas Fusarium oxysporum f.sp. canariensis1
Fusarium oxysporum f.sp. cannabis Fusarium oxysporum f.sp. cattleyae1
Fusarium oxysporum f.sp. cepae1 Fusarium oxysporum f.sp. chrysanthemi
Fusarium oxysporum f.sp. ciceris1 Fusarium oxysporum f.sp. cubense
Fusarium oxysporum f.sp. cucumerinum1 Fusarium oxysporum f.sp. cyclaminis
Fusarium oxysporum f.sp. dianthi1 Fusarium oxysporum f.sp. fragariae
Fusarium oxysporum f.sp. gladioli1 Fusarium oxysporum f.sp. koae1
Fusarium oxysporum f.sp. lactucae1 Fusarium oxysporum f.sp. lentis1
Fusarium oxysporum f.sp. lilli Fusarium oxysporum f.sp. lini
Fusarium oxysporum f.sp. lycopersici Fusarium oxysporum f.sp. medicaginis1
Fusarium oxysporum f.sp. melonis Fusarium oxysporum f.sp. narcissi1
Fusarium oxysporum f.sp. nicotianae1 Fusarium oxysporum f.sp. niveum1
Fusarium oxysporum f.sp. palmarum1 Fusarium oxysporum f.sp. passiflorae
Fusarium oxysporum f.sp. phaseoli1 Fusarium oxysporum f.sp. pisi1
Fusarium oxysporum f.sp. radicis-lycospersici Fusarium oxysporum f.sp. tuberosi1
Fusarium oxysporum f.sp. tuliape1 Fusarium oxysporum f.sp. vasinfectum
Fusarium oxysporum f.sp. vasinfectum1 Fusarium oxysporum f.sp. zingiberi1
1Predicted detection by in silico analysis only

Formae Speciales Not Detected
Fusarium oxysporum f.sp. betae

Cross-reactions
Fusarium sporotrichioides1
1A weak cross reaction was observed inconsistently.

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents