AmplifyRP® XRT for Xf (Xylella fastidiosa)
Tech Note: New Xf Test Format
AmplifyRP XRT for Xf is a rapid DNA amplification and detection platform designed for testing almond, blueberry, citrus, grapevine, and olive for Xylella fastidiosa. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Xf DNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for Xf
- PD1 Pellet diluent
- 1.5 mL microcentrifuge tubes
- AMP1 extraction buffer
- Sample extraction bags, mesh
- User Guide
- Tech Note
Required but Not Included:
- 10 µL Pipette
- 25 µL Pipette
- 1 mL Pipette
- 10 µL, 25 µL, and 1 mL Barrier Pipette Tips
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT | Full Validation Report |
| Analytical Sensitivity | |
|---|---|
| Analytical Sensitivity: | The assay is 66.7% sensitive between 100 ag/µL and 10 ag/µL. (n=12) |
| Limit of detection: | The assay has a 100% detection rate at 100 ag/µL with DNA fragments. (n=6) |
| The assay has a 33.3% detection rate at 10 ag/µL with DNA fragments. (n=6) | |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| Xf subsp. fastidiosa | Xf subsp. multiplex |
| Xf subsp. pauca | Xf subsp. sandyi |
| Xf subsp. temecula | |
| Cross-reactions | |
|---|---|
| None known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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