AmplifyRP® XRT+ for Xf (Xylella fastidiosa)
Test Label: Biotin labeled primer / FAM probe
Test Format: AmplifyRP® XRT+
Xylella fastidiosa (Xf) is the causal agent of disease in multiple crops including Pierce's disease in grape, citrus variegated chlorosis (CVC), almond leaf scorch disease and olive quick decline syndrome. Severe Xf infections can decimate entire crops and cause enormous financial losses to growers.
AmplifyRP XRT+ for Xf is an isothermal DNA amplification and detection system that offers unrivaled detection capabilities in an easy-to-use testing format. It is highly specific and offers equivalent, or better, sensitivity as published PCR methods while eliminating laborious and costly nucleic acid extractions. Prior molecular diagnostic experience is not required to perform AmplifyRP XRT+ tests.
XRT+ is our most flexible AmplifyRP platform and offers the option of either real-time detection (Image 2) or end-point detection (Image 3). Real-time detection is completed using the field deployable (battery operated) AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
If you need a printable barcode for this assay, click here.
End-point detection can alternatively be completed by performing the amplification step in a small 42°C heat block followed by amplicon detection using an Amplicon detection chamber (sold separately).
Limit of Detection (LOD): 22 copies of Xf genomic DNA per reaction.
Specificity: No known cross-reactions to other pathogens
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay. Less than 45 minutes when used with the Amplicon Detection Chamber as an end-point assay.
- XRT+ reaction pellets for Xf (48)
- PD1 Pellet diluent (55 mL)
- Amp1 extraction buffer (3 x 55 mL)
- Micro centrifuge tubes (50)
- Mesh extraction bags (50)
- User Guide
If you plan to use this assay as a real-time assay you will need to purchase the following items separately:
- AmpliFire Isothermal Fluorometer
If you plan to use this assay as a end-point detection assay you will need to purchase the following items separately:
- AmplifyRP Acceler8 starter pack
- Amplicon detection chambers
- Disposable transfer pipettes (optional if you don't have laboratory pipettes)