AmplifyRP® XRT for Cms (Clavibacter michiganensis subsp. sepedonicus)
Tech Note: New Cms Test Format
AmplifyRP® XRT for Cms is a rapid DNA amplification and detection platform designed for testing potato tubers for Clavibacter michiganensis subsp. sepedonicus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Cms DNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for Cms
- Pre-filled 100 µL PD1 Pellet diluent tubes
- AMP1 extraction buffer
- User Guide
- Tech Note
Required but Not Included:
- 5 µL Pipette
- 25 µL Pipette
- 1 mL Pipette
- 5 µL, 25 µL, and 1 mL Barrier Pipette Tips
- Microcentrifuge Tubes, 1.5 mL
- 4 mil ply plastic bags (or equivalent)
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT | Full Validation Report |
| Analytical Sensitivity | |
|---|---|
| Analytical Sensitivity: | The assay is 95.0% sensitive between 100 fg/µL and 10 fg/µL. (n=20) |
| Limit of detection: | The assay has a 100% detection rate at 100 fg/µL with DNA fragement. (n=10) |
| The assay has a 90% detection rate at 10 fg/µL with DNA fragement. (n=10) | |
| Analytical Sensitivity: | The assay is 87.5% sensitive between 105 CFU and 104 CFU. (n=8) |
| Limit of detection: | The assay has a 100% detection rate at 105 CFU with Cms bacteria. (n=4) |
| The assay has a 75% detection rate at 104 CFU with Cms bacteria. (n=4) | |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| Cms-2 (INM) (ID, USA) | Cms-AS-1 (MN, USA) |
| Cms-BCP45 | Cms-BCP45 (RifR mutation) |
| Cms-CIC4 | Cms-CIC77 |
| Cms-CIC8 | Cms-CS R8 |
| Cms-CS101 (Canada) (ATCC® 33113™) | Cms-Cs2 |
| Cms-Cs2 (RifR mutation) | Cms-Cs3 (Canada) |
| Cms-Cs3 (RifR mutation) | Cms-Cs3-1 |
| Cms-Cs3-2 | Cms-Cs3M |
| Cms-Cs3NM (avirulent) | Cms-Cs3NM (virulent) |
| Cms-Cs3RC | Cms-Cs4 |
| Cms-Cs5 (NY, USA) (ATCC® 9850™) | Cms-Cs7 |
| Cms-Cs7 (RifR mutation) | Cms-Cs9 |
| Cms-CsR8 | Cms-DGBBC 235 |
| Cms-P45 (lacks pCS1) | Cms-R5 |
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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