AmplifyRP® XRT+ for Cms (Clavibacter michiganensis subsp. sepedonicus)
AmplifyRP® XRT+ for Cms is a rapid DNA amplification and detection platform designed for end-point or real-time detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers and bacterial culture. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Cms DNA a single operating temperature (39 °C).
Clavibacter michiganensis subsp. sepedonicus (Cms) is the causal agent of bacterial ring rot in potato and is a zero tolerance pathogen in US and Canadian seed certification programs. Infected tuber lots exhibit range of symptoms depending on the level of infection, the cultivar, and environment. One of the most classical visual symptoms in tubers is decaying vascular ring tissue which may be evident when the tuber is cut. Cms is most commonly spread through contaminated seed cutting tools and/or farm equipment.
AmplifyRP® XRT+ is a flexible platform offering both end-point and real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
Additional items required for detection:
Real-time detection: this assay requires a fluorometer like the battery operated AmpliFire® fluorometer for real-time detection. When using the AmpliFire®, assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
End-point detection: Amplicon Detection Chambers must be used for end-point detection. Amplicon detection chambers consist of a lateral flow strip inside a contamination-proof chamber. The lateral flow strip detects dual labeled (FAM & Biotin) DNA / RNA amplicons. Visual results interpretation is similar to that of ImmunoStrips®.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT+ tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
- XRT reaction pellets for Cms
- Pre-filled 100 µL PD1 Pellet diluent tubes
- AMP1 Extraction Buffer
- User Guide
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT+
|Limit of detection: Approximately 1 copy/µL of plasmid DNA fragments|
|Limit of detection: Approximately 20 fg/µL of genomic DNA fragments|
|Isolate Names / Geographic Strain Locations|
|Cms-AS-1 (MN, USA)||Cms-BCP45|
|Cms-BCP45 (RifR mutation)||Cms-CIC4|
|Cms-CS R8||Cms-CS101 (Canada) (ATCC® 33113™)|
|Cms-Cs2||Cms-Cs2 (RifR mutation)|
|Cms-Cs3 (Canada)||Cms-Cs3 (RifR mutation)|
|Cms-Cs4||Cms-Cs5 (NY, USA) (ATCC® 9850™)|
|Cms-Cs7||Cms-Cs7 (RifR mutation)|
|Cms-P45 (lacks pCS1)||Cms-R5|
For a list of validated hosts, please see the Full Validation Report.