AmplifyRP® XRT for WCLaV-1,2 (Watermelon crinkle leaf-associated virus 1 & 2)
AmplifyRP® XRT for WCLaV-1,2 is a rapid RNA amplification and detection platform designed for testing cucurbits for Watermelon crinkle leaf-associated virus 1 & 2. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify WCLaV-1,2 RNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for WCLaV-1,2
- Pre-filled 100 µL PD1 Pellet diluent tubes
- GEB2-filled mesh extraction bags
- User Guide
Required but Not Included:
- 5 µL Pipette
- 25 µL Pipette
- 5 and 25 µL Barrier Pipette Tips
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
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Test Format: AmplifyRP® XRT |
Full Validation Report |
| Analytical Sensitivity | ||
|---|---|---|
| WCLaV-1 | Analytical Sensitivity: | The assay is 81.3% sensitive between 500 ag/µL and 1 fg/µL. (n=16) |
| Limit of Detection: | The assay has a 100% detection rate at 1 fg/µL with RNA transcripts. (n=8) | |
| The assay has a 62.5% detection rate at 500 ag/µL with RNA transcripts. (n=8) | ||
| WCLaV-2 | Analytical Sensitivity: | The assay is 75.0% sensitive between 10 fg/µL and 100 fg/µL. (n=32) |
| Limit of Detection: | The assay has a 100% detection rate at 100 fg/µL with RNA transcripts. (n=8) | |
| The assay has a 50.0% detection rate at 10 fg/µL with RNA transcripts. (n=24) | ||
| Isolate Names / Geographic Strain Locations | |
|---|---|
| WCLaV-1 USA isolate | WCLaV-1-Ju-01 (Brazil)1 |
| WCLaV-1-KF-1 (China)1 | WCLaV-1-S6 (Australia)1 |
| WCLaV-2 USA isolate | WCLaV-2-Ju-01 (Brazil)1 |
| WCLaV-2-KF-15 (China)1 | |
1Predicted detection by in silico analysis only
| Cross-reactions | |
|---|---|
| Virus Name | Species Name |
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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| Option | Unit | Purchase |
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AmplifyRP XRT for WCLaV-1,2, 8 reactions
XCS 39800/0008
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Shown in cart
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AmplifyRP XRT for WCLaV-1,2, 48 reactions
XCS 39800/0048
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Shown in cart
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AmplifyRP XRT for WCLaV-1,2, 96 reactions
XCS 39800/0096
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Shown in cart
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