AmplifyRP® XRT for ToBRFV (Tomato brown rugose fruit virus)

Part Number:
XCS 66800/0048
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Host Reaction: See Validation Report regarding host reactions with the internal control observed with pepper samples. An alternative AmpliFire® QR code for pepper tissue is provided in the User Guide or here.

AmplifyRP® XRT for ToBRFV is a rapid RNA amplification and detection platform designed for testing peppers and tomatoes for Tomato brown rugose fruit virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify ToBRFV RNA and an endogenous RNA control at a single operating temperature (42 °C).

AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.

The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.

Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.

If you would prefer to add a field-deployable serological assay to your testing toolkit, please check out Agdia's ToBRFV ImmunoStrips®.

Click here for other product documents such as the User Guide or printable barcode.

Included:

  • XRT reaction pellets for ToBRFV
  • Pre-filled 100 µL PD1 Pellet diluent tubes
  • GEB-filled mesh extraction bags
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: ROX-labeled control probe (endogenous)

Full Validation Report

Host Reaction: See Validation Report regarding host reactions with the internal control observed with pepper samples. An alternative AmpliFire® QR code for pepper tissue is provided in the User Guide or here.

Analytical Sensitivity
Limit of detection: Approximately 15 fg/µL of RNA transcript

Isolate Names / Geographic Strain Locations
ToBRFV Italy isolate ToBRFV Israel isolate
ToBRFV Mexico isolate ToBRFV USA isolate

Cross-reactions
None known1
1Does not cross-react with any other Tobamovirus species.

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents