AmplifyRP® XRT for ToBRFV (Tomato brown rugose fruit virus)
Test Label: FAM-labeled target probe
Test Format: AmplifyRP® XRT
Internal Control: Yes - Endogenous
AmplifyRP® XRT for ToBRFV is a rapid RNA amplification and detection platform designed for testing peppers and tomatoes for Tomato brown rugose fruit virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify ToBRFV RNA and an endogenous RNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests.
NOTE: This assay requires a fluorometer to work properly.
If you need a printable barcode for this assay, click here.
Limit of Detection (LOD): Approximately 15 fg/μL of RNA transcript.
- Inclusive: Designed to detect all ToBRFV isolates.
- Exclusive: Does not cross-react with any other Tobamovirus species.
Host Limitations: Some pepper varieties may not amplify the internal control. An alternative AmpliFire® QR code for pepper tissue is provided in the user guide.
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay.