Host Reaction: See Validation Report regarding host reactions with the internal control observed with pepper samples. An alternative AmpliFire^® QR code for pepper tissue is provided in the User Guide or here.

AmplifyRP^® XRT for ToBRFV is a rapid RNA amplification and detection platform designed for testing peppers and tomatoes for Tomato brown rugose fruit virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify ToBRFV RNA and an endogenous RNA control at a single operating temperature (42 °C).

AmplifyRP^® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.

The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.

Prior molecular diagnostic experience is not required to perform AmplifyRP^® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.

If you would prefer to add a field-deployable serological assay to your testing toolkit, please check out Agdia's ToBRFV ImmunoStrips®.

Click here for other product documents such as the User Guide or printable barcode.