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ImmunoStrip® for Tomato brown rugose fruit virus (ToBRFV)

The Tomato brown rugose fruit virus (ToBRFV) ImmunoStrip® is used to detect the presence of ToBRFV in solanaceous crops. ImmunoStrips® are the perfect screening tool for use in the field, greenhouse, and the lab.

The ToBRFV ImmunoStrip® cross-reacts with Tomato mottle mosaic virus (ToMMV) and has mild cross-reactivity to Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV). For more technical information, click here.

The ToBRFV ImmunoStrip® is estimated to have up to a threefold increase in sensitivity for ToBRFV compared to Agdia's TMV ImmunoStrip®.

Tomato brown rugose fruit virus is an extremely stable virus and can easily infect adjacent plants and contaminate surfaces such as benches, pots, tools, etc.

If you would prefer to add a field-deployable molecular assay to your testing toolkit, please check out Agdia's ToBRFV AmplifyRP® XRT method.

Click here for other product documents such as the User Guide or Validation Report.

Included in ISK Kit:

Included in STX Item:

Required but Not Included:

  • Scissors
  • Extraction tool
  • Letter holder or other device to hold sample extraction bags upright

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: ImmunoStrip® Lateral Flow Device (LFD)
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 64 - 320 pg/mL of purified virus
Limit of detection: 1:259,200 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ToBRFV-VE484 (DSM 29970) ToBRFV PV-1236 (Germany)
ToBRFV PV-1241 (Israel) ToBRFV PV-1244 (Germany)
ToBRFV PV-1278 (Netherlands) ToBRFV PV-1300 (Cyprus)
ToBRFV (Italy isoate 1) ToBRFV (Italy isolate 2)
ToBRFV (Mexico)

Tobacco mosaic virus (TMV)1 Tomato mosaic virus (ToMV)1
Tomato mottle mosaic virus (ToMMV)

1Mild cross-reactivity observed. Detection limit greater than 200 ng/mL.

Does not cross-react with
Bell Pepper Mottle Virus (BPeMV) Chili pepper mild mottle virus (CPMMoV)
Cucumber green mottle mosaic virus (CGMMV) Kyuri green mottle mosaic virus (KGMMV)
Pepper mild mottle virus (PMMoV) Piper chlorosis virus (PChV)1,2
Tobacco mild green mosaic virus (TMGMV) Zucchini Green Mottle Mosaic Virus (ZGMMV)
1Confirmed experimentally at Agdia, Inc.
2Reported to not detect Piper chlorosis virus (PChV), a possible novel Tobamovirus.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
ImmunoStrip® for ToBRFV, 5 strips and buffer filled bags
ISK 66800/0005
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ImmunoStrip® for ToBRFV, 25 strips and buffer filled bags
ISK 66800/0025
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STX 66800/0025
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