ImmunoStrip® for Tomato brown rugose fruit virus (ToBRFV)

The Tomato brown rugose fruit virus (ToBRFV) ImmunoStrip® is used to detect the presence of ToBRFV in solanaceous crops. ToBRFV ImmunoStrips® are perfect for use in the field, greenhouse, and the lab.

The ToBRFV ImmunoStrip® cross-reacts with Tomato mottle mosaic virus (ToMMV) and has mild cross-reactivity to Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV). For more technical information, click here.

The ToBRFV ImmunoStrip® is estimated to have up to a threefold increase in sensitivity for ToBRFV compared to Agdia's TMV ImmunoStrip®

Tomato brown rugose fruit virus is an extremely stable virus and can easily infect adjacent plants and contaminate surfaces such as benches, pots, tools, etc

User Guide

Validation Report Summary

Test Format: ImmunoStrip®
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal


Analytical Sensitivity
Limit of Detection: Between 6-32 pg/mL of purified viral particles
Isolate Names / Geographic Strain Locations
ToBRFV PV1236 (Germany) ToBRFV PV1241 (Israel)
ToBRFV PV1278 (Netherlands) ToBRFV (Italy isolate 1)
ToBRFV (Italy isolate 2) ToBRFV (Mexico)
Cross-Reactivity
Cross-reacts with: Tomato mottle mosaic virus (ToMMV) and has mild cross-reactivity to Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV).
Did not cross-react with the following Tobamoviruses during product validation testing: Bell Pepper Mottle Virus (BPeMV), Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), Pepper mild mottle virus (PMMoV), Tobacco mild green mosaic virus (TMGMV), and Zucchini Green Mottle Mosaic Virus (ZGMMV).

Kit (ISK) Includes:

  • ImmunoStrips®
  • Sample bags containing SEB1 buffer
  • User Guide

ImmunoStrips® Only (STX) Includes:

  • ImmunoStrips®
  • SEB1 extraction buffer purchased separately
Option Unit Purchase
ImmunoStrip® for ToBRFV, 5 strips and buffer filled bags
ISK 66800/0005
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ImmunoStrip® for ToBRFV, 25 strips and buffer filled bags
ISK 66800/0025
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ImmunoStrip® for ToBRFV ***BUFFER FILLED BAGS NOT INCLUDED***
STX 66800/0025
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