AmplifyRP® XRT for PVY (Potato virus Y)
Test Label: FAM-labeled target probe
Test Format: AmplifyRP® XRT
Internal Control: Yes - Endogenous
AmplifyRP XRT for PVY is a rapid RNA amplification and detection platform designed for testing potatoes and ornamentals for Potato virus Y. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify PVY RNA and an endogenous RNA control at a single operating temperature (42 °C).
AmplifyRP XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP XRT tests.
NOTE: This assay requires a fluorometer to work properly.
If you need a printable barcode for this assay, click here.
Limit of Detection (LOD): Approximately 10 fg/μL of RNA transcripts
Specificity:
- Inclusive: Detects only PVY.
- Exclusive: Does not cross-react with other closely related viruses.
Host Limitations: None known.
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay.
Includes:
- XRT reaction pellets for PVY (48)
- Pre-filled 100 µL PD1 Pellet diluent tubes (48)
- GEB-filled mesh extraction bags (50)
- User Guide
NOTE: This assay requires a fluorometer to work properly.
If you need a printable barcode for this assay, click here.