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AmplifyRP® XRT for PVY (Potato virus Y)

Part Number:
XCS 20001/0048
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AmplifyRP® XRT for PVY is a rapid RNA amplification and detection platform designed for testing potatoes and ornamentals for Potato virus Y. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify PVY RNA and an endogenous RNA control at a single operating temperature (42 °C).

AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.

The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.

Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.

If you would prefer to add a field-deployable serological assay to your testing toolkit, please check out Agdia's PVY ImmunoStrips®.

Click here for other product documents such as the User Guide or printable barcode.

Included:

  • XRT reaction pellets for PVY
  • Pre-filled 100 µL PD1 Pellet diluent tubes
  • GEB-filled mesh extraction bags
  • User Guide

Required but Not Included:

Optional:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: ROX-labeled control probe (endogenous)

Full Validation Report

Analytical Sensitivity
Limit of detection: Approximately 10 fg/µL of RNA transcripts

Isolate Names / Geographic Strain Locations
PVYC PVYN
PVYN:O PVYNaN
PVYNN PVYNTN
PVYN-Wi PVYO
PVYO+C PVYO5
PVYC-E48 (Iran)1 PVYC-PRI-509 (Netherlands)1
PVY-LYE84.2 (Spain)1 PVYN:O-A95 (USA)1
PVYN:O-Alt1 PVYN:O-LW (Poland)1
PVYN-6051 PVYNaN-Nicola (Germany)1
PVYNaN-RRA-11 PVYNaN-Tu 6601
PVYNE11-ID20 (USA)1 PVYNE11-NE111
PVYN-Mont1 PVYNTN-156 (Germany)1
PVYNTN-HR1 (USA)1 PVYNTN-PB312 (USA)1
PVYN-Wi-LR (USA)1 PVYN-Wi-N1 (USA)1
PVYN-Wi-PN10A1 PVYO5-ID269 (USA)1
PVYO5-ID968 (USA)1 PVYO5-ME56 (USA)1
PVYO-ME173 (USA)1 PVYO-Oz (USA)1
PVYO-SCRI-O (UK)1 PVY-SON41 (France)1

1Predicted detection by in silico analysis only


Cross-reactions
Virus Name Species Name
None Known

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents