AmplifyRP® XRT for Pram (Phytophthora ramorum)
AmplifyRP® XRT for Pram is a rapid DNA amplification and detection platform designed for testing ornamental plants for Phytophthora ramorum. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Pram DNA and an endogenous DNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
48 Pellet Size
Included:
- XRT reaction pellets for Pram
- Pre-filled 100 µL PD1 Pellet diluent tubes
- GEB2-filled mesh extraction bags
- User Guide
Required but Not Included:
- 5 µL Pipette
- 25 µL Pipette
- 5 and 25 µL Barrier Pipette Tips
- AmpliFire® or equivalent fluorometer
Optional:
96-well High-Throughput Format: (Coming Soon)
Included:
- 96-well XRT reaction plate for Pram
- Pre-filled 100 µL PD1 Pellet diluent tubes
- General extract buffer 2, GEB2 powder
- User Guide
Required but Not Included:
- 5 µL Pipette
- 25 µL Pipette
- 5 and 25 µL Barrier Pipette Tips
- Mesh Sample Bags
- Real-time PCR (qPCR) Instrument1
Optional:
1Agdia’s high-throughput assay is validated for use with the Bio-rad® CFX96™ and Applied Biosciences™ QuantStudio™ 3 and 5 Real-Time (qPCR) machines. Other real-time instruments may also be compatible for use with this product but have not been evaluated.
For a full list of included items, including sizes, please see the Content List.
Bio-Rad® is a registered trademark of Bio-Rad Laboratories, Inc. CFX96™ is a trademark of Bio-Rad Laboratories, Inc. Applied Biosystems™ and QuantStudio™ are trademarks of Life Technologies Corporation.Reference to any products, services, hypertext link to third parties or other information by trade name, trademark, supplier or otherwise does not constitute or imply its endorsement, sponsorship or recommendation by Agdia, Inc. or its contributors/employees.
Validation Report Summary
Test Format: AmplifyRP® XRT | Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: Approximately 1 fg/µL of plasmid DNA fragments |
| Limit of detection: Approximately 100 fg/µL of whole genomic DNA fragments |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| Pram-EU1 | Pram-EU2 |
| Pram-NA1 | Pram-NA2 |
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
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| Option | Unit | Purchase |
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AmplifyRP XRT for Pram, 48 reactions (6 strips of 8 pellets)
XCS 93400/0048
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Shown in cart
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AmplifyRP XRT for Pram, 96 reactions (1 plate of 96 pellets)
XCS 93400/0096
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Shown in cart
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