AmplifyRP® XRT for PMTV (Potato mop-top virus)
Test Label: FAM-labeled target probe
Test Format: AmplifyRP® XRT
Internal Control: Yes - Endogenous
AmplifyRP XRT for PMTV is a rapid RNA amplification and detection platform designed for testing potato leaves, stems, tubers, or tissue culture plantlets for Potato mop-top virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify PMTV RNA and an endogenous RNA control at a single operating temperature (42 °C).
AmplifyRP XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP XRT tests.
NOTE: This assay requires a fluorometer to work properly.
If you need a printable barcode for this assay, click here.
Limit of Detection (LOD): Approximately 10 fg/μL of RNA transcripts
- Inclusive: Detects only PMTV.
- Exclusive: Does not cross-react with other closely related viruses.
Host Limitations: Not recommended for sprout testing.
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay.