AmplifyRP® XRT for BPYV & SPaV (Beet pseudoyellows virus and Strawberry pallidosis-associated virus)
AmplifyRP® XRT for BPYV & SPaV is a rapid RNA amplification and detection platform designed for testing cucurbits, ornamentals, strawberries, and other vegetable crops for Beet pseudoyellows virus and Strawberry pallidosis-associated virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify and differentiate BPYV & SPaV RNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for BPYV & SPaV
- Pre-filled 100 µL PD1 Pellet diluent tubes
- GEB2-filled mesh extraction bags
- User Guide
Required but Not Included:
- 5 µL Pipette
- 25 µL Pipette
- 5 and 25 µL Barrier Pipette Tips
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
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Test Format: AmplifyRP® XRT |
Full Validation Report |
| Analytical Sensitivity | ||
|---|---|---|
| Analytical Sensitivity varies depending on which viral species is present in the sample. | ||
| BPYV | Analytical Sensitivity: | The assay is 81.3% sensitive between 100 fg/µL and 300 fg/µL. (n=16) |
| Limit of Detection: | The assay has a 100% detection rate at 300 fg/µL with RNA transcripts. (n=8) | |
| The assay has a 62.5% detection rate at 100 fg/µL with RNA transcripts. (n=8) | ||
| SPaV | Analytical Sensitivity: | The assay is 68.8% sensitive between 10 fg/µL and 100 fg/µL. (n=16) |
| Limit of Detection: | The assay has a 100% detection rate at 100 fg/µL with RNA transcripts. (n=8) | |
| The assay has a 37.5% detection rate at 10 fg/µL with RNA transcripts. (n=8) | ||
| Isolate Names / Geographic Strain Locations | |
|---|---|
| BPYV-MI (Japan)1 | SPaV-FJ (China)1 |
| SPaV-M1 (MD, USA) | SPaV-SPaV/H2435 (USA)1 |
1Predicted detection by in silico analysis only
| Cross-reactions | |
|---|---|
| Virus Name | Species Name |
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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| Option | Unit | Purchase |
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AmplifyRP XRT for BPYV & SPaV, 8 reactions
XCS 87000/0008
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Shown in cart
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AmplifyRP XRT for BPYV & SPaV, 48 reactions
XCS 87000/0048
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Shown in cart
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AmplifyRP XRT for BPYV & SPaV, 96 reactions
XCS 87000/0096
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Shown in cart
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