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PathoScreen® Kit for Tomato brown rugose fruit virus (ToBRFV)

Tech Note: ToBRFV ELISA Protocol and Performance Summary

This ELISA test is a qualitative serological assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper leaf and seed.

Tomato brown rugose fruit virus is a resistance-breaking Tobamovirus that causes severe economic losses in solanaceous crops, including Solanum lycopersicum (tomato) and Capsicum spp. (pepper). It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit. These symptoms decrease yield and render fruit unmarketable.

Click here for other product documents such as the User Guide or Buffer Instructions.


Required but Not Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 64 - 320 pg/mL of purified virus
Limit of detection: 1:259,200 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ToBRFV-VE484 (DSM 29970) ToBRFV PV-1236 (Germany)
ToBRFV PV-1241 (Israel) ToBRFV PV-1244 (Germany)
ToBRFV PV-1278 (Netherlands) ToBRFV (Italy isoate 1)
ToBRFV (Italy isolate 2) ToBRFV (Mexico)

Tobacco mosaic virus (TMV)1 Tomato mosaic virus (ToMV)1,2
Tomato mottle mosaic virus (ToMMV)

1Very low cross-reactivity was observed with a limited number of high titer samples.
2Cross-reactivity observed with one seed sample when utilizing the Agdia method; cross-reactivity not observed when testing using the infectious tobamovirus tomato seed ISHI method with a 1X PBS extraction buffer.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tomato brown rugose fruit virus (ToBRFV), 96 testwells
PSA 66800/0096
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PathoScreen® Kit, Tomato brown rugose fruit virus (ToBRFV), 288 testwells
PSA 66800/0288
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PathoScreen® Kit, Tomato brown rugose fruit virus (ToBRFV), 480 testwells
PSA 66800/0480
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