Tech Note: ToBRFV ELISA Protocol and Performance Summary

This ELISA test is a qualitative serological assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper leaf and seed.

Tomato brown rugose fruit virus is a resistance-breaking Tobamovirus that causes severe economic losses in solanaceous crops, including Solanum lycopersicum (tomato) and Capsicum spp. (pepper). It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit. These symptoms decrease yield and render fruit unmarketable.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ToBRFV ImmunoStrips®

Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.

See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.

• Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
• PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
• Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
• Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.