ELISA Reagent Set for Tomato brown rugose fruit virus (ToBRFV)
Tech Note: ToBRFV ELISA Protocol and Performance Summary
This ELISA test is a qualitative serological assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper leaf and seed.
Tomato brown rugose fruit virus is a resistance-breaking Tobamovirus that causes severe economic losses in solanaceous crops, including Solanum lycopersicum (tomato) and Capsicum spp. (pepper). It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit. These symptoms decrease yield and render fruit unmarketable.
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For a full list of included items, including sizes, please see the Content List.
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Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
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| Limit of detection: 64 - 320 pg/mL of purified virus |
| Limit of detection: 1:259,200 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
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| ToBRFV-VE484 (DSM 29970) | ToBRFV PV-1236 (Germany) |
| ToBRFV PV-1241 (Israel) | ToBRFV PV-1244 (Germany) |
| ToBRFV PV-1278 (Netherlands) | ToBRFV (Italy isoate 1) |
| ToBRFV (Italy isolate 2) | ToBRFV (Mexico) |
| Cross-reactions | |
|---|---|
| Tobacco mosaic virus (TMV)1 | Tomato mosaic virus (ToMV)1,2 |
| Tomato mottle mosaic virus (ToMMV) | |
1Very low cross-reactivity was observed with a limited number of high titer samples.
2Cross-reactivity observed with one seed sample when utilizing the Agdia method; cross-reactivity not observed when testing using the infectious tobamovirus tomato seed ISHI method with a 1X PBS extraction buffer.
For a list of validated hosts, please see the Full Validation Report.
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Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 96 testwells
SRA 66800/0096
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Shown in cart
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Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 500 testwells
SRA 66800/0500
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Shown in cart
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Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 1000 testwells
SRA 66800/1000
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Shown in cart
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Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 5000 testwells
SRA 66800/5000
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Shown in cart
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