ELISA Test Kits for ToBRFV (Tomato brown rugose fruit virus)
Tech Note: ToBRFV ELISA Protocol and Performance Summary
This ELISA test is a qualitative serological assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper leaf and seed.
Tomato brown rugose fruit virus is a resistance-breaking Tobamovirus that causes severe economic losses in solanaceous crops, including Solanum lycopersicum (tomato) and Capsicum spp. (pepper). It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit. These symptoms decrease yield and render fruit unmarketable.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ToBRFV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
- Tech Note
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
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Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 64 - 320 pg/mL of purified virus |
| Limit of detection: 1:259,200 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| ToBRFV Italy isolate 1 | ToBRFV Italy isolate 2 |
| ToBRFV Mexico isolate | ToBRFV PV-1236 (Germany) |
| ToBRFV PV-1241 (Israel) | ToBRFV PV-1244 (Germany) |
| ToBRFV PV-1278 (Netherlands) | ToBRFV PV-1300 (Cyprus) |
| ToBRFV-VE484 (DSM 29970) | |
| Cross-reactions | |
|---|---|
| Virus Name | Species Name |
| Paprika mild mottle virus (PaMMV)3 | Tobamovirus paprikae |
| Tobacco mosaic virus (TMV)1 | Tobamovirus tabaci |
| Tomato mosaic virus (ToMV)1,2 | Tobamovirus tomatotessellati |
| Tomato mottle mosaic virus (ToMMV) | Tobamovirus maculatessellati |
1Very low cross-reactivity was observed with a limited number of high titer samples.
2Cross-reactivity observed with one seed sample when utilizing the Agdia method;
cross-reactivity not observed when testing using the infectious tobamovirus tomato seed ISHI method with a 1X
PBS extraction buffer.
31 out of 3 isolates of Paprika mild mottle virus (PaMMV) were detected.
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
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ToBRFV Reagent Set, 96 Testwells
SRA 66800/0096
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Shown in cart
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ToBRFV Reagent Set, 500 Testwells
SRA 66800/0500
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Shown in cart
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ToBRFV Reagent Set, 1000 Testwells
SRA 66800/1000
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Shown in cart
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ToBRFV Reagent Set, 5000 Testwells
SRA 66800/5000
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Shown in cart
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ToBRFV PathoScreen® Complete Kit, 96 Testwells
PSA 66800/0096
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Shown in cart
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ToBRFV PathoScreen® Complete Kit, 288 Testwells
PSA 66800/0288
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Shown in cart
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ToBRFV PathoScreen® Complete Kit, 480 Testwells
PSA 66800/0480
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Shown in cart
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