ELISA Reagent Set for Tomato brown rugose fruit virus (ToBRFV)

Tech Note: ToBRFV ELISA Protocol and Performance Summary

This ELISA test is a qualitative serological assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper leaf and seed.

Tomato brown rugose fruit virus is a resistance-breaking Tobamovirus that causes severe economic losses in solanaceous crops, including Solanum lycopersicum (tomato) and Capsicum spp. (pepper). It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit. These symptoms decrease yield and render fruit unmarketable.

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

Required but Not Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
  • Tech Note

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:259,200 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ToBRFV PV-1236 (Germany) ToBRFV PV-1241 (Israel)
ToBRFV PV-1278 (Netherlands) ToBRFV (Italy isolate 1)
ToBRFV (Italy isolate 2) ToBRFV (Mexico)

Cross-reactions
Very low cross-reactivity was observed with a limited number of high titer TMV and ToMV samples. Cross-reactivity was not observed with any other Tobamoviruses

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 96 testwells
SRA 66800/0096
Shown in cart
Sign in to Buy In Stock
Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 500 testwells
SRA 66800/0500
Shown in cart
Sign in to Buy In Stock
Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 1000 testwells
SRA 66800/1000
Shown in cart
Sign in to Buy In Stock
Reagent Set for Tomato brown rugose fruit virus (ToBRFV), 5000 testwells
SRA 66800/5000
Shown in cart
Sign in to Buy In Stock