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PathoScreen® Kit for Tobacco etch virus (TEV)

This ELISA test is a qualitative serological assay for the detection of Tobacco etch virus (TEV) in ornamental and vegetable leaves. TEV is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TEV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:64,800 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
TEV-HAT (Highly Aphid Transmitted) TEV-Mex21 (Mexico)
TEV-N (USA) TEV-NAT (Not Aphid Transmitted)
TEV PV-69 (WI, USA) TEV PV-364

Pepper mottle virus (PepMoV) Pepper veinal mottle virus (PVMV)
Potato virus Y (PVY)1 Potato virus Y-necrotic strain (PVY-n)2
Tobacco vein mottling virus (TVMV)

1Cross-reaction observed with PVY in 2 out of 4 samples with limited data set, including samples of PVY-n.
2Cross-reaction observed with PVY-n in 1 out of 2 samples with limited data set.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tobacco etch virus (TEV), 96 testwells
PSA 49501/0096
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PathoScreen® Kit, Tobacco etch virus (TEV), 288 testwells
PSA 49501/0288
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PathoScreen® Kit, Tobacco etch virus (TEV), 480 testwells
PSA 49501/0480
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