PathoScreen® Kit for Potyvirus Group (Poty)

Agdia's Potyvirus Group test detects most aphid-transmitted members of the Potyvirus Group. This test is based on a monoclonal antibody (PTY 1) developed by the USDA Florist and Nursery Crops Laboratory in Beltsville, Maryland, USA. The broad reactivity of this antibody is possible because it reacts to a sequence of amino acids on the virus coat protein which is highly conserved among aphid-transmitted potyviruses. This test is intended to be used as a general screening tool. We recommend confirmation of positive results by another method (PCR, etc.)

Potyviruses are the largest group of plant pathogens and affect hundreds of crops. Viruses within this genus are transmitted through one or more of the following means: aphid, seed, mechanical, graft and pollen. Potyviruses can have a significant impact on crop production including loss of yield, unmarketable product and regulatory impacts.

Host Reaction: Scaevola tissue

Test Label: Alkaline Phosphatase
Test Format: Antigen coated plate (ACP) ELISA
Capture Reagent: Polystyrene Plate
Detection Reagent: Monoclonal

The list below represents Potyviruses that have been shown to be detected by the Potyvirus group ELISA test and does not represent all viruses that may be detected. If you have confirmed detection of a Potyvirus not on this list, please contact us. We would like to work with you to further validate the Potyvirus group ELISA detection capabilities.

Alstroemeria streak virus (AlStV) Omphalodes virus Y (OmVY)
Angelica virus Y (AnVY) Onion yellow dwarf virus (OYDV)
Asparagus virus 1 (AV-1) Ornithologum mosaic virus (OrMV)
Basella rugose mosaic virus (BaRMV) Papaya ringspot virus (PRSV)¹
Bean common mosaic virus (BCMV) Passionfruit woodiness virus (PWV)
Bean yellow mosaic virus (BYMV) Pea mosaic virus (PMV)
Beet mosaic virus (BtMV) Pea seedborne mosaic virus (PSbMV)
Begonia flower breaking virus (BFBV)** Peanut mottle virus (PeMoV)²
Blackeye cowpea mosaic virus (BICMV) Pepper mottle virus (PepMoV)
Carnation vein mottle virus (CVMV) Plum pox virus (PPV)
Celery mosaic virus (CeMV) Pokeweed mosaic virus (PkMV)
Chickpea yellow mosaic virus (CpYMV) Potato virus A (PVA)
Chilli vein banding mottle virus (CVbMV) Potato virus V (PVV)
Clover yellow vein virus (ClYVV)¹ Potato virus Y (PVY)
Columbian datura virus (CDV) Ryegrass mosaic virus (RgMV)³
Cowpea aphid-borne mosaic virus (CABMV) Soybean mosaic virus (SMV)
Dasheen mosaic virus (DsMV) Spiranthes mosaic virus 2 (SpiMV 2)
Euphorbia ringspot virus (EuRV) Statice virus Y (StaVY)
Freesia mosaic virus (FreMV) Sugarcane mosaic virus (SCMV)
Garlic mosaic virus (GarMV) Sunflower mosaic virus (SuMV)
Hyacinth mosaic virus (HyMV) Sweet potato feathery mottle virus (SPFMV)
Impatiens flower break virus (IFBV) Sweet potato latent virus (SPLV)
Iris mild mosaic virus (IMMV) Tobacco etch virus (TEV)
Iris severe mosaic virus (ISMV) Tobacco vein mottling virus (TVMV)
Johnsongrass mosaic virus (JgMV)* Tricyrtis virus Y (TrVY)
Konjak mosaic virus (KoMV) Tuberose mild mosaic virus (TMMV)
Leek yellow stripe virus (LYSV) Tulip breaking virus (TBV)
Lettuce mosaic virus (LMV) Tulip chlorotic blotch virus (TCBV)
Maize dwarf mosaic virus (MDMV) Turnip mosaic virus (TuMV)
Malva vein clearing virus (MVCV) Vallota mosaic virus (ValMV)
Meadow saffron breaking virus (MSBV) Watermelon mosaic virus (WMV)
Murraya koenigii potyvirus White lupin mosaic virus (WLMV)
Narcissus late seasons yellows virus (NLSYV) Yam mild mosaic virus (YMMV)
Narcissus yellow stripe virus (NYSV) Yam mosaic virus (YMV)
Nerine yellow stripe virus (NeYSV) Zucchini yellow mosaic virus (ZYMV)

1Most isolates detected

2Some isolates detected

3RgMV is a Rymovirus

*A weak reaction was observed with in-house isolates of JgMV. This pathogen may not be reliably detected with low titer samples.

**BFBV has been reported to be detected.

Includes:

  • Detection antibody
  • Alkaline phosphatase enzyme conjugate
  • Indirect sample extraction buffer, 10X concentrate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • 96 well microtiter plate, strip
  • PBST buffer, 20X concentrate, 50ml
  • User Guide
  • Buffer Instructions
  • Logsheet
Option Unit Purchase
PathoScreen® Kit, Potyvirus group (Poty), 96 testwells
PSA 27200/0096
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PathoScreen® Kit, Potyvirus group (Poty), 288 testwells
PSA 27200/0288
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PathoScreen® Kit, Potyvirus group (Poty), 480 testwells
PSA 27200/0480
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