PathoScreen® Kit for Potyvirus Group (Poty)

Host Reaction: See Validation Report regarding host reactions observed with Scaevola and cucurbit samples.

Agdia's Potyvirus Group test detects most aphid-transmitted members of the Potyvirus Group. This test is based on a monoclonal antibody (PTY 1) developed by the USDA Florist and Nursery Crops Laboratory in Beltsville, Maryland, USA. The broad reactivity of this antibody is possible because it reacts to a sequence of amino acids on the virus coat protein which is highly conserved among aphid-transmitted potyviruses. This test is intended to be used as a general screening tool. We recommend confirmation of positive results by another method (PCR, etc.)

Potyviruses are the largest group of plant pathogens and affect hundreds of crops. Viruses within this genus are transmitted through one or more of the following means: aphid, seed, mechanical, graft and pollen. Potyviruses can have a significant impact on crop production including loss of yield, unmarketable product and regulatory impacts.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Poty ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Detection antibody
  • Alkaline phosphatase enzyme conjugate
  • Indirect sample extraction buffer, 10X concentrate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • PBST buffer, 20X concentrate, 50 mL
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Antigen coated plate (ACP) ELISA
Capture Reagent: Polystyrene Plate
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:194,400 dilution of infected tissue (pathogen titer unknown)

Potyviruses Detected1
Alstroemeria streak virus (AlStV) Angelica virus Y (AnVY)
Apium virus Y (ApVY)2 Asparagus virus 1 (AV-1)
Basella rugose mosaic virus (BaRMV) Bean common mosaic virus (BCMV)
Bean yellow mosaic virus (BYMV) Beet mosaic virus (BtMV)
Begonia flower breaking virus (BFBV)3 Blackeye cowpea mosaic virus (BlCMV)
Brugmansia mosaic virus (BruMV) Brugmansia suaveolens mottle virus (BsMoV)
Carnation vein mottle virus (CVMV) Celery mosaic virus (CeMV)
Chickpea yellow mosaic virus (CpYMV) Chilli vein banding mottle virus (CVbMV)
Colombian datura virus (CDV) Clover yellow vein virus (ClYVV)4
Cowpea aphid-borne mosaic virus (CABMV) Dasheen mosaic virus (DsMV)
Euphorbia ringspot virus (EuRV) Freesia mosaic virus (FreMV)
Garlic mosaic virus (GarMV) Hippeastrum mosaic virus (HiMV)5
Hyacinth mosaic virus (HyMV) Impatiens flower break virus (IFBV)
Iris mild mosaic virus (IMMV) Iris severe mosaic virus (ISMV)
Johnsongrass mosaic virus (JGMV)6 Konjak mosaic virus (KoMV)
Leek yellow stripe virus (LYSV) Lettuce mosaic virus (LMV)
Maize dwarf mosaic virus (MDMV) Malva vein clearing virus (MVCV)
Meadow saffron breaking virus (MSBV) Murraya koenigii potyvirus
Muscari mosaic virus Narcissus late seasons yellows virus (NLYSV)
Narcissus latent virus (NLV)5 Narcissus mosaic virus (NMV)5
Narcissus yellow stripe virus (NYSV) Nerine yellow stripe virus (NeYSV)
Omphalodes virus Y (OmVY) Onion yellow dwarf virus (OYDV)
Ornithologum mosaic virus (OrMV) Papaya ringspot virus (PRSV)7
Passionfruit woodiness virus (PWV) Pea mosaic virus (PMV)
Pea seedborne mosaic virus (PSbMV) Peanut mottle virus (PeMoV)8
Pepper mottle virus (PepMoV) Plum pox virus (PPV)
Pokeweed mosaic virus (PkMV) Potato virus A (PVA)
Potato virus V (PVV) Potato virus Y (PVY)
Soybean mosaic virus (SMV) Spiranthes mosaic virus 2 (SpiMV 2)
Statice virus Y (StaVY) Sugarcane mosaic virus (SCMV)
Sunflower mosaic virus (SuMV) Sweet potato feathery mottle virus (SPFMV)
Sweet potato latent virus (SPLV) Tobacco etch virus (TEV)
Tobacco vein mottling virus (TVMV) Tricyrtis virus Y (TrVY)
Tuberose mild mosaic virus (TMMV) Tulip breaking virus (TBV)
Tulip chlorotic blotch virus (TCBV) Turnip mosaic virus (TuMV)
Vallota mosaic virus (ValMV) Watermelon mosaic virus (WMV)
White lupin mosaic virus (WLMV) Yam mild mosaic virus (YMMV)
Yam mosaic virus (YMV) Zucchini yellow mosaic virus (ZYMV)

1The list above represents Potyviruses that have been shown to be detected by the Potyvirus group ELISA test and does not represent all viruses that may be detected. If you have confirmed detection of a Potyvirus not on this list, please contact us. We would like to work with you to further validate the Potyvirus group ELISA detection capabilities.

2Apium virus Y (ApVY) is reported to be detected.
3Begonia flower breaking virus (BFBV) is reported to be detected.
4Most isolates of Clover yellow vein virus (ClYVV) are detected.
5Hippeastrum mosaic virus (HiMV), Narcissus latent virus (NLV), and Narcissus mosaic virus (NMV) have been reported to be detected.
6Weak reaction observed with in-house isolates of JGMV. May not be reliably detected with low titer samples.
7Most isolates of Papaya ringspot virus (PRSV) are detected.
8Some isolates of Peanut mottle virus (PeMoV) are detected.

Cross-reactions
Ryegrass mosaic virus (RgMV)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Potyvirus group (Poty), 96 testwells
PSA 27200/0096
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PathoScreen® Kit, Potyvirus group (Poty), 288 testwells
PSA 27200/0288
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PathoScreen® Kit, Potyvirus group (Poty), 480 testwells
PSA 27200/0480
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