ImmunoStrip® for Potyvirus Group (Poty)

Agdia's Poty ImmunoStrip can quickly identify more than 40 members of the Potyvirus genus and can be used to test more than 55 different crops. The test can be used in the field or in the lab and requires no experience to perform. Results are available in as little as 5 minutes with a maximum run time of 30 minutes.

Agdia's Potyvirus Group test detects most aphid-transmitted members of the Potyvirus Group. This test is based on a monoclonal antibody (PTY 1) developed by the USDA Florist and Nursery Crops Laboratory in Beltsville, Maryland, USA. The broad reactivity of this antibody is possible because it reacts to a sequence of amino acids on the virus coat protein which is highly conserved among aphid-transmitted potyviruses. This test is intended to be used as a general screening tool. We recommend confirmation of positive results by another method (PCR, etc.).

Potyviruses are the largest group of plant pathogens and affect hundreds of crops. Viruses within this genus are transmitted through one or more of the following means: aphid, seed, mechanical, graft and pollen. Potyviruses can have a significant impact on crop production including loss of yield, unmarketable product and regulatory impacts.

Click here for other product documents such as the User Guide or Validation Report.

Included in ISK Kit:

Included in STX Item:

Required but Not Included:

  • Scissors
  • Extraction tool
  • Letter holder or other device to hold sample extraction bags upright

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: ImmunoStrip® Lateral Flow Device (LFD)
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal and Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:3,645 dilution of infected tissue (pathogen titer unknown)

Potyviruses Detected1
Alstroemeria mosaic virus (AlMV) Asparagus virus 1 (AV-1)
Bean common mosaic necrosis virus (BCMNV) Bean common mosaic virus (BCMV)2
Bean yellow mosaic virus (BYMV) Beet mosaic virus (BtMV)
Begonia flower breaking virus (BFBV)3 Brugmansia mosaic virus (BruMV)
Brugmansia suaveolens mottle virus (BsMoV) Catharanthus mosaic virus (CatMV)
Celery mosaic virus (CeMV) Chilli veinal mottle virus (ChiVMV)
Clover yellow vein virus (ClYVV) Dasheen mosaic virus (DsMV)
Euphorbia ringspot virus (EuRSV) Freesia mosaic virus (FreMV)
Hippeastrum mosaic virus (HiMV) Hyacinth mosaic virus (HyaMV)
Iris severe mosaic virus (ISMV) Johnsongrass mosaic virus (JGMV)2
Kalanchoe mosaic virus (KMV) Leek yellow stripe virus (LYSV)
Lettuce mosaic virus (LMV) Lily mottle virus (LMoV)
Maize dwarf mosaic virus (MDMV) Moroccan watermelon mosaic virus (MWMV)
Muscari mosaic virus (MuMV) Narcissus late season yellows virus (NLSYV)
Narcissus yellow stripe virus (NYSV) Onion yellow dwarf virus (OYDV)
Papaya ringspot virus (PRSV) Pea seed-borne mosaic virus (PSbMV)
Peanut mottle virus (PeMoV) Pepper mottle virus (PepMoV)
Pepper veinal mottle virus (PVMV) Pepper yellow mosaic virus (PepYMV)
Plum pox virus (PPV) Pokeweed mosaic virus (PkMV)
Potato virus A (PVA) Potato virus V (PVV)
Potato virus Y (PVY) Ranunculus mild mosaic virus (RanMMV)
Shallot yellow stripe virus (SYSV) Soybean mosaic virus (SMV)
Spiranthes mosaic virus 3 (SpiMV-3) Sugarcane mosaic virus (SCMV)
Sweet potato feathery mottle virus (SPFMV) Sweet potato virus 2 (SPV-2)
Sweet potato virus C (SPVC) Sweet potato virus G (SPVG)
Tobacco etch virus (TEV) Tobacco vein mottling virus (TVMV)
Tomato necrotic stunt virus (ToNStV) Tuberose mild mosaic virus (TuMMV)
Tulip breaking virus (TBV) Turnip mosaic virus (TuMV)
Washington tulip virus (WaTV) Watermelon mosaic virus (WMV)
Zantedeschia mosaic virus (ZaMMV) Zucchini yellow mosaic virus (ZYMV)
1The list above represents Potyviruses that have been shown to be detected by the Potyvirus group ImmunoStrip test and does not represent all viruses that may be detected. If you have confirmed detection of a Potyvirus not on this list, please contact us. We would like to work with you to further validate the Potyvirus group ImmunoStrip detection capabilities.
2A weak reaction was observed with in-house isolates of JGMV. Not all samples of BCMV were detected. These pathogens may not be reliably detected with low titer samples.
3Begonia flower breaking virus (BFBV) is reported to be detected.

Potyviruses Not Detected
Basella rugose mosaic virus (BaRMV)1
1One sample of BaRMV was not detected; further samples were unavailable for confirmation.
Cross-reactions
None known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
ImmunoStrip® for Potyvirus Group (Poty), 5 strips & buffer filled bags
ISK 27200/0005
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ImmunoStrip® for Potyvirus Group (Poty), 25 strips & buffer filled bags
ISK 27200/0025
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ImmunoStrips® only for Potyvirus Group (Poty) ***BUFFER FILLED BAGS NOT INCLUDED***
STX 27200/0025
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