ImmunoStrip® for Potyvirus Group (Poty)
Tech Note: Product Improvement
Agdia's Poty ImmunoStrip® can quickly identify more than 20 members of the Potyvirus genus and can be used to test more than 70 different crops. The test can be used in the field or in the lab and requires no experience to perform. Results are available in as little as 5 minutes with a maximum run time of 30 minutes.
Agdia's Potyvirus Group test includes the use of monoclonal antibody (PTY 1) developed by the USDA Florist and Nursery Crops Laboratory in Beltsville, Maryland, USA. The broad reactivity of this antibody is possible because it reacts to a sequence of amino acids on the virus coat protein which is highly conserved among aphid-transmitted potyviruses. This test is intended to be used as a general screening tool. We recommend confirmation of positive results by another method (PCR, etc.).
Potyviruses are the largest group of plant pathogens and affect hundreds of crops. Viruses within this genus are transmitted through one or more of the following means: aphid, seed, mechanical, graft and pollen. Potyviruses can have a significant impact on crop production including loss of yield, unmarketable product and regulatory impacts.
Click here for other product documents such as the User Guide or Validation Report.
Included in ISK Kit:
- ImmunoStrips®
- Sample bags containing SEB1 buffer
- User Guide
- Tech Note
Included in STX Item:
- ImmunoStrips®
- User Guide
- Tech Note
Required but Not Included:
- Scissors
- Extraction tool
- Agdia tissue homogenizer, marker, or pen
- Letter holder or other device to hold sample extraction bags upright
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: ImmunoStrip® Lateral Flow Device (LFD) |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection (PVY): 1:500 dilution of infected tissue (pathogen titer unknown) |
| Limit of detection (TEV): 1:2,500 dilution of infected tissue (pathogen titer unknown) |
| Limit of detection (ZYMV): 1:500 dilution of infected tissue (pathogen titer unknown) |
| Potyviruses Detected1 | |
|---|---|
| Alstroemeria mosaic virus (AlMV) | Bean common mosaic virus (BCMV) |
| Bean yellow mosaic virus (BYMV) | Kalanchoe mosaic virus (KMV) |
| Leek yellow stripe virus (LYSV) | Lettuce mosaic virus (LMV) |
| Maize dwarf mosaic virus (MDMV) | Moroccan watermelon mosaic virus (MWMV) |
| Onion yellow dwarf virus (OYDV) | Papaya ringspot virus (PRSV) |
| Pea seed borne-mosaic virus (PSbMV) | Peanut mottle virus (PeMoV) |
| Pepper mottle virus (PepMoV) | Potato virus V (PVV) |
| Potato virus Y (PVY) | Soybean mosaic virus (SMV) |
| Sugarcane mosaic virus (SCMV) | Sweet potato feathery mottle virus (SPFMV) |
| Tobacco etch virus (TEV) | Tobacco vein mottling virus (TVMV) |
| Tomato necrotic stunt virus (TNSV) | Turnip mosaic virus (TuMV) |
| Watermelon mosaic virus (WMV) | Zucchini yellow mosaic virus (ZYMV) |
| 1The list above represents Potyviruses that have been shown to be detected by the Potyvirus group ImmunoStrip test and does not represent all viruses that may be detected. If you have confirmed detection of a Potyvirus not on this list, please contact us. We would like to work with you to further validate the Potyvirus group ImmunoStrip detection capabilities. | |
| For a full list of the Poty ImmunoStrip detections and non-detections, please see the Full Validation Report. | |
| Cross-reactions | |
|---|---|
| None known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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| Option | Unit | Purchase |
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ImmunoStrip® for Poty, 5 strips and buffer filled bags
ISK 27200/0005
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Shown in cart
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ImmunoStrip® for Poty, 25 strips and buffer filled bags
ISK 27200/0025
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Shown in cart
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ImmunoStrip® for Poty ***BUFFER FILLED BAGS NOT INCLUDED***
STX 27200/0025
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Shown in cart
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