ELISA Test Kits for Poty (Potyvirus Group)
Host Reaction: See Validation Report regarding host reactions observed with Scaevola and cucurbit samples.
Agdia's Potyvirus Group test detects most aphid-transmitted members of the Potyvirus Group. This test is based on a monoclonal antibody (PTY 1) developed by the USDA Florist and Nursery Crops Laboratory in Beltsville, Maryland, USA. The broad reactivity of this antibody is possible because it reacts to a sequence of amino acids on the virus coat protein which is highly conserved among aphid-transmitted potyviruses. This test is intended to be used as a general screening tool. We recommend confirmation of positive results by another method (PCR, etc.)
Potyviruses are the largest group of plant pathogens and affect hundreds of crops. Viruses within this genus are transmitted through one or more of the following means: aphid, seed, mechanical, graft and pollen. Potyviruses can have a significant impact on crop production including loss of yield, unmarketable product and regulatory impacts.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Poty ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Detection antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00444)
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- Indirect sample extraction buffer, powder
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Detection antibody
- Alkaline phosphatase enzyme conjugate
- Indirect sample extraction buffer, 10X concentrate
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- 96-well microtiter plates, solid* (*strip in single plate set)
- PBST buffer, 20X concentrate, 50 mL
- User Guide
Required but Not Included:
Coating Antibody (Format not available)
Enzyme Conjugate
Included in Enzyme Conjugate:
- Detection antibody
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:194,400 dilution of infected tissue (pathogen titer unknown) |
| Potyviruses Detected1 | |
|---|---|
| Alstroemeria mosaic virus (AlMV)2 | Angelica virus Y (AnVY) |
| Apium virus Y (ApVY)3 | Asparagus virus 1 (AV-1) |
| Basella rugose mosaic virus (BaRMV) | Bean common mosaic virus (BCMV)4 |
| Bean common mosaic necrosis virus (BCMNV) | Bean yellow mosaic virus (BYMV)21,22 |
| Beet mosaic virus (BtMV) | Begonia flower breaking virus (BFBV)5 |
| Brugmansia mosaic virus (BruMV) | Brugmansia suaveolens mottle virus (BsMoV) |
| Carnation vein mottle virus (CVMV) | Celery mosaic virus (CeMV) |
| Chickpea yellow mosaic virus (CpYMV)19 | Chilli veinal mottle virus (ChiVMV)6 |
| Clover yellow vein virus (ClYVV)7,8 | Colombian datura virus (CDV) |
| Cowpea aphid-borne mosaic virus (CABMV) | Dasheen mosaic virus (DsMV) |
| Euphorbia ringspot virus (EuRV) | Freesia mosaic virus (FreMV)9 |
| Hippeastrum mosaic virus (HiMV)10 | Hyacinth mosaic virus (HyMV) |
| Impatiens flower break virus (IFBV) | Iris mild mosaic virus (IMMV) |
| Iris severe mosaic virus (ISMV) | Johnsongrass mosaic virus (JGMV)11 |
| Konjac mosaic virus (KoMV) | Leek yellow stripe virus (LYSV)12 |
| Lettuce mosaic virus (LMV) | Maize dwarf mosaic virus (MDMV) |
| Malva vein clearing virus (MVCV) | Meadow saffron breaking virus (MSBV) |
| Murraya koenigii potyvirus13 | Muscari mosaic virus19 |
| Narcissus late season yellows virus (NLYSV) | Narcissus yellow stripe virus (NYSV) |
| Nerine yellow stripe virus (NeYSV) | Omphalodes virus Y (OmVY)19 |
| Onion yellow dwarf virus (OYDV) | Ornithogalum mosaic virus (OrMV) |
| Papaya ringspot virus (PRSV)14 | Passion fruit woodiness virus (PWV) |
| Pea seed-borne mosaic virus (PSbMV) | Peanut mottle virus (PeMoV)15 |
| Pepper mottle virus (PepMoV) | Pepper yellow mosaic virus (PepYMV)16 |
| Plum pox virus (PPV) | Pokeweed mosaic virus (PkMV) |
| Potato virus A (PVA) | Potato virus V (PVV) |
| Potato virus Y (PVY) | Ranunculus mild mosaic virus (RanMMV) |
| Soybean mosaic virus (SMV) | Spiranthes mosaic virus 3 (SpiMV 3)17 |
| Sugarcane mosaic virus (SCMV) | Sunflower mosaic virus (SuMV) |
| Sweet potato feathery mottle virus (SPFMV) | Sweet potato latent virus (SPLV) |
| Tobacco etch virus (TEV) | Tobacco vein mottling virus (TVMV) |
| Tricyrtis virus Y (TrVY)19 | Tuberose mild mosaic virus (TMMV) |
| Tulip breaking virus (TBV) | Turnip mosaic virus (TuMV)18 |
| Vallota mosaic virus (ValMV) | Watermelon mosaic virus (WMV) |
| Yam mild mosaic virus (YMMV) | Yam mosaic virus (YMV) |
| Zucchini yellow mosaic virus (ZYMV) | |
1The list above represents Potyviruses that have been shown to be detected by the Potyvirus group ELISA test and does not represent all viruses that may be detected. If you have confirmed detection of a Potyvirus not on this list, please contact us. We would like to work with you to further validate the Potyvirus group ELISA detection capabilities.
2Alstroemeria streak virus (AlStV) is now known as Alstroemeria mosaic virus (AlMV).
3Apium virus Y (ApVY) is reported to be detected.
4Blackeye cowpea mosaic virus (BlCMV) is now known as Bean common mosaic virus (BCMV).
5Begonia flower breaking virus (BFBV) is reported to be detected.
6Chilli vein-banding mottle virus (CVbMV) is now known as Chilli veinal mottle virus (ChiVMV).
7Most isolates of Clover yellow vein virus (ClYVV) are detected.
8Statice virus Y (StaVY) is now known as Clover yellow vein virus (ClYVV).
9Spiranthes mosaic virus 2 (SpiMV 2) is now known as Freesia mosaic virus (FreMV).
10Hippeastrum mosaic virus (HiMV) is reported to be detected.
11Weak reaction observed with in-house isolates of JGMV. May not be reliably detected with low titer samples.
12Garlic mosaic virus (GarMV) is now known as Leek yellow stripe virus (LYSV).
13Murraya koenigii potyvirus, a tentatively proposed potyvirus, is reported to be detected.
14Most isolates of Papaya ringspot virus (PRSV) are detected.
15Some isolates of Peanut mottle virus (PeMoV) are detected.
16Pepper yellow mosaic virus (PepYMV) is reported to be detected.
17Spiranthes mosaic virus 3 (SpiMV 3) is reported to be detected.
18Tulip chlorotic blotch virus (TCBV) is now known as Turnip mosaic virus (TuMV).
19Related, unclassified virus.
20Zucchini tigre mosaic virus has been reported to be detected via western blot utilzing Agdia’s antibody.
21White lupin mosaic virus (WLMV) is now known as Bean yellow mosaic virus (BYMV).
22Pea mosaic virus (PMV) is now known as Bean yellow mosaic virus (BYMV).
| Cross-reactions | |
|---|---|
| Ryegrass mosaic virus (RgMV) | |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
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Poty Reagent Set, 96 Testwells
SRA 27200/0096
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Shown in cart
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Poty Reagent Set, 500 Testwells
SRA 27200/0500
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Shown in cart
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Poty Reagent Set, 1000 Testwells
SRA 27200/1000
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Shown in cart
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Poty Reagent Set, 5000 Testwells
SRA 27200/5000
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Shown in cart
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Poty PathoScreen® Complete Kit, 96 Testwells
PSA 27200/0096
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Shown in cart
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Poty PathoScreen® Complete Kit, 288 Testwells
PSA 27200/0288
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Shown in cart
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Poty PathoScreen® Complete Kit, 480 Testwells
PSA 27200/0480
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Shown in cart
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