ELISA Reagent Set for Potyvirus Group (Poty)
Host Reaction: See Validation Report regarding host reactions observed with Scaevola and cucurbit samples.
Agdia's Potyvirus Group test detects most aphid-transmitted members of the Potyvirus Group. This test is based on a monoclonal antibody (PTY 1) developed by the USDA Florist and Nursery Crops Laboratory in Beltsville, Maryland, USA. The broad reactivity of this antibody is possible because it reacts to a sequence of amino acids on the virus coat protein which is highly conserved among aphid-transmitted potyviruses. This test is intended to be used as a general screening tool. We recommend confirmation of positive results by another method (PCR, etc.)
Potyviruses are the largest group of plant pathogens and affect hundreds of crops. Viruses within this genus are transmitted through one or more of the following means: aphid, seed, mechanical, graft and pollen. Potyviruses can have a significant impact on crop production including loss of yield, unmarketable product and regulatory impacts.
Click here for other product documents such as the User Guide or Buffer Formulations.
- Detection antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00444)
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- Indirect sample extraction buffer, powder
- Buffer Formulations from Chemicals
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Label: Alkaline Phosphatase
Full Validation Report
|Limit of detection: 1:194,400 dilution of infected tissue (pathogen titer unknown)|
|Alstroemeria mosaic virus (AlMV)2||Angelica virus Y (AnVY)|
|Apium virus Y (ApVY)3||Asparagus virus 1 (AV-1)|
|Basella rugose mosaic virus (BaRMV)||Bean common mosaic virus (BCMV)4|
|Bean yellow mosaic virus (BYMV)21,22||Beet mosaic virus (BtMV)|
|Begonia flower breaking virus (BFBV)5||Brugmansia mosaic virus (BruMV)|
|Brugmansia suaveolens mottle virus (BsMoV)||Carnation vein mottle virus (CVMV)|
|Celery mosaic virus (CeMV)||Chickpea yellow mosaic virus (CpYMV)19|
|Chilli veinal mottle virus (ChiVMV)6||Clover yellow vein virus (ClYVV)7,8|
|Colombian datura virus (CDV)||Cowpea aphid-borne mosaic virus (CABMV)|
|Dasheen mosaic virus (DsMV)||Euphorbia ringspot virus (EuRV)|
|Freesia mosaic virus (FreMV)9||Hippeastrum mosaic virus (HiMV)10|
|Hyacinth mosaic virus (HyMV)||Impatiens flower break virus (IFBV)|
|Iris mild mosaic virus (IMMV)||Iris severe mosaic virus (ISMV)|
|Johnsongrass mosaic virus (JGMV)11||Konjac mosaic virus (KoMV)|
|Leek yellow stripe virus (LYSV)12||Lettuce mosaic virus (LMV)|
|Maize dwarf mosaic virus (MDMV)||Malva vein clearing virus (MVCV)|
|Meadow saffron breaking virus (MSBV)||Murraya koenigii potyvirus13|
|Muscari mosaic virus19||Narcissus late season yellows virus (NLYSV)|
|Narcissus yellow stripe virus (NYSV)||Nerine yellow stripe virus (NeYSV)|
|Omphalodes virus Y (OmVY)19||Onion yellow dwarf virus (OYDV)|
|Ornithogalum mosaic virus (OrMV)||Papaya ringspot virus (PRSV)14|
|Passion fruit woodiness virus (PWV)||Pea seed-borne mosaic virus (PSbMV)|
|Peanut mottle virus (PeMoV)15||Pepper mottle virus (PepMoV)|
|Pepper yellow mosaic virus (PepYMV)16||Plum pox virus (PPV)|
|Pokeweed mosaic virus (PkMV)||Potato virus A (PVA)|
|Potato virus V (PVV)||Potato virus Y (PVY)|
|Ranunculus mild mosaic virus (RanMMV)||Soybean mosaic virus (SMV)|
|Spiranthes mosaic virus 3 (SpiMV 3)17||Sugarcane mosaic virus (SCMV)|
|Sunflower mosaic virus (SuMV)||Sweet potato feathery mottle virus (SPFMV)|
|Sweet potato latent virus (SPLV)||Tobacco etch virus (TEV)|
|Tobacco vein mottling virus (TVMV)||Tricyrtis virus Y (TrVY)19|
|Tuberose mild mosaic virus (TMMV)||Tulip breaking virus (TBV)|
|Turnip mosaic virus (TuMV)18||Vallota mosaic virus (ValMV)|
|Watermelon mosaic virus (WMV)||Yam mild mosaic virus (YMMV)|
|Yam mosaic virus (YMV)||Zucchini yellow mosaic virus (ZYMV)|
1The list above represents Potyviruses that have been shown to be detected by the Potyvirus group ELISA test and does not represent all viruses that may be detected. If you have confirmed detection of a Potyvirus not on this list, please contact us. We would like to work with you to further validate the Potyvirus group ELISA detection capabilities.
2Alstroemeria streak virus (AlStV) is now known as Alstroemeria mosaic virus (AlMV).
3Apium virus Y (ApVY) is reported to be detected.
4Blackeye cowpea mosaic virus (BlCMV) is now known as Bean common mosaic virus (BCMV).
5Begonia flower breaking virus (BFBV) is reported to be detected.
6Chilli vein-banding mottle virus (CVbMV) is now known as Chilli veinal mottle virus (ChiVMV).
7Most isolates of Clover yellow vein virus (ClYVV) are detected.
8Statice virus Y (StaVY) is now known as Clover yellow vein virus (ClYVV).
9Spiranthes mosaic virus 2 (SpiMV 2) is now known as Freesia mosaic virus (FreMV).
10Hippeastrum mosaic virus (HiMV) is reported to be detected.
11Weak reaction observed with in-house isolates of JGMV. May not be reliably detected with low titer samples.
12Garlic mosaic virus (GarMV) is now known as Leek yellow stripe virus (LYSV).
13Murraya koenigii potyvirus, a tentatively proposed potyvirus, is reported to be detected.
14Most isolates of Papaya ringspot virus (PRSV) are detected.
15Some isolates of Peanut mottle virus (PeMoV) are detected.
16Pepper yellow mosaic virus (PepYMV) is reported to be detected.
17Spiranthes mosaic virus 3 (SpiMV 3) is reported to be detected.
18Tulip chlorotic blotch virus (TCBV) is now known as Turnip mosaic virus (TuMV).
19Related, unclassified virus.
20Zucchini tigre mosaic virus has been reported to be detected via western blot utilzing Agdia’s antibody.
21White lupin mosaic virus (WLMV) is now known as Bean yellow mosaic virus (BYMV).
22Pea mosaic virus (PMV) is now known as Bean yellow mosaic virus (BYMV).
|Ryegrass mosaic virus (RgMV)|
For a list of validated hosts, please see the Full Validation Report.
Reagent Set for Potyvirus Group (Poty), 96 testwells
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