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PathoScreen® Kit for Impatiens necrotic spot virus (INSV)

This ELISA test is a qualitative serological assay for the detection of Impatiens necrotic spot virus (INSV) in fruit, ornamental, and vegetable leaves. INSV is a member of the Orthotospovirus genus known for their enveloped, spherical-shaped virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's INSV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Detection antibody, Bottle A
  • Alkaline phosphatase enzyme conjugate, Bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound ELISA
Capture Reagent: Monoclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:12,960 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of INSV. Twenty distinct samples of INSV have been experimentally proven to be detected.

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.