AmplifyRP® XRT+ for Dickeya spp.

Part Number:
XCS 55500/0048
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Test Label: Biotin labeled primer / FAM probe
Test Format: AmplifyRP® XRT+

AmplifyRP XRT+ for Dickeya spp. is an isothermal DNA amplification and detection system that rapidly amplifies small portions of DNA conserved among all Dickeya spp.

XRT+ is our most flexible AmplifyRP platform and offers the option of either real-time detection (Image 1) or end-point detection (Image 2). Real-time detection is completed using the field deployable (battery operated) AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).

End-point detection can alternatively be completed by performing the amplification step in a small 39°C heat block followed by amplicon detection using an Amplicon Detection Chamber (sold separately).

Prior molecular diagnostic experience is not required to perform AmplifyRP XRT+ tests.

Limit of Detection (LOD): ~ 2 x 102 cfu / mL

Specificity:

  • Inclusive: Dickeya chrysanthemi, Dickeya dianthicola, Dickeya diffenbachiae, Dickeya solani, Dickeya zeae
  • Exclusive: Does not react with: Pectobacterium spp, Clavibacter michiganensis subsp. sepidonicus, Ralstonia solanacearum, Fusarium oxysporum Race 4 or Race 8, Phytophthora megasperma, Phytophthora cryptogea, Phytophthora irregularis, Phytophthora infestans, Phytophthora drechsleri, Phytophthora erythroseptica, Pseudomonas fluoresens, or Candidatus Liberibacter solanacearum

Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay. Less than 45 minutes when used with the Amplicon Detection Chamber as an end-point assay.

  • Technical Information
  • Includes
  • Includes:

    • XRT+ reaction pellets for Dickeya spp. (48)
    • Pre-filled 100 µL PD1 Pellet diluent tubes (48)
    • Amp1 extraction buffer (55 mL)
    • User Guide
    If you plan to use this assay as a real-time assay you will need to purchase the following items separately:
    • AmpliFire Isothermal Fluorometer
    If you plan to use this assay as a end-point detection assay you will need to purchase the following items separately:
    • AmplifyRP Acceler8 starter pack
    • Amplicon detection chambers
    • Disposable transfer pipettes (optional if you don't have laboratory pipettes)