AmplifyRP® XRT for TRV (Tobacco rattle virus)
AmplifyRP® XRT for TRV is a rapid RNA amplification and detection platform designed for testing potato tubers and ornamentals for Tobacco rattle virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify TRV RNA and an endogenous RNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT
|Limit of detection: Approximately 100 fg/µL of RNA transcripts|
|Isolate Names / Geographic Strain Locations|
|TRV PV-0043||TRV-M (Oregon mild) (OR, USA) (PV-0351) (ATCC® PV-72™)|
|TRV-Oregon severe (OR, USA) (PV-0350) (ATCC® PV-64™)||TRV-Oregon yellow (OR, USA) (PV-0352) (ATCC® PV-73™)|
|TRV-Potato ring necrosis (United Kingdom) (PV-0354) (ATCC® PV-526™)||TRV-SYM (Spinach Yellow Mottle) (ATCC® PV-525™) (England)|
For a list of validated hosts, please see the Full Validation Report.