AmplifyRP® XRT for ToMMV (Tomato mottle mosaic virus)
AmplifyRP® XRT for ToMMV is a rapid RNA amplification and detection platform designed for testing peppers and tomatoes for Tomato mottle mosaic virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify ToMMV RNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for ToMMV
- Pre-filled 100 µL PD1 Pellet diluent tubes
- GEB2-filled mesh extraction bags
- User Guide
Required but Not Included:
- 5 µL Pipette
- 25 µL Pipette
- 5 and 25 µL Barrier Pipette Tips
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT | Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: Approximately 400 ag/µL of RNA transcript |
| Limit of detection: 1:62,500 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| ToMMV PV-1267 (CA, USA) | ToMMV PV-1342 (Mauritius)1 |
| ToMMV-10-100 (FL, USA)1 | ToMMV-19-02305 (Netherlands)1 |
| ToMMV-CA16-01 (USA)1 | ToMMV-CpB1 (Brazil)1 |
| ToMMV-Hainan (China)1 | ToMMV-HN (China)1 |
| ToMMV-Hn18 (China)1 | ToMMV-Hn19 (China)1 |
| ToMMV-Hn23 (China)1 | ToMMV-LN (China)1 |
| ToMMV-MX5 (Mexico) | ToMMV-NVWA 36783676 (Netherlands)1 |
| ToMMV-NVWA36783860 (China)1 | ToMMV-NVWA411068131 |
| ToMMV-NVWA57856601 | ToMMV-NY-13 (USA)1 |
| ToMMV-SC13-05 (USA)1 | ToMMV-SY11026 (China)1 |
| ToMMV-TiLhaLJ (China)1 | ToMMV-ToMMV_83 (Viet Nam)1 |
| ToMMV-VLC-1 (Spain)1 | ToMMV-WD-YMZ1 (China)1 |
| ToMMV-YYMFQ12 (China)1 | ToMMV-YYMLJ (China)1 |
1Predicted detection by in silico analysis only
| Cross-reactions | |
|---|---|
| None Known1 | |
1Does not cross-react with any other Tobamovirus species.
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
|---|---|---|
|
|
|
|
|
|
|
|
||