AmplifyRP® XRT for HLVd (Hop latent viroid)
Tech Note: Test Improvement and Protocol Change
AmplifyRP® XRT for HLVd is a rapid RNA amplification and detection platform designed for field-based or laboratory testing of hops and Cannabis spp (including industrial hemp) for Hop latent viroid. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify HLVd RNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT
|Limit of detection: Approximately 1 fg/µL of RNA transcripts|
|Limit of detection: 1:20,000 dilution of infected tissue (pathogen titer unknown)|
|Isolate Names / Geographic Strain Locations||Analytical Sensitivity|
|HLVd Michigan isolate (MI, USA)||Unknown|
|HLVd-BoulderHLVd (CO, USA)1||Unknown|
|HLVd-CR2 (CA, USA)1||Unknown|
|HLVd-CR3A (CA, USA)1||Unknown|
|HLVd-CV_12 (USA)||Approximately 100 fg/µL of RNA transcripts|
|HLVd-CV_38 (USA)||Approximately 1 fg/µL of RNA transcripts|
|HLVd-CV_81 (USA)||Approximately 1 fg/µL of RNA transcripts|
|HLVd-CV_88 (USA)||Approximately 10 fg/µL of RNA transcripts|
|HLVd-CV_93 (USA)||Approximately 1 fg/µL of RNA transcripts|
|HLVd-DeltaHLVd (CO, USA)1||Unknown|
|HLVd-H2 (China)||Approximately 1 fg/µL of RNA transcripts|
|HLVd-HpLVd001 (CA, USA)1||Unknown|
|HLVd-RefSeq (Germany)||Approximately 1 fg/µL of RNA transcripts|
|HLVd-T229 (Czech Republic)1||Unknown|
|HLVd-T50 (Czech Republic)1||Unknown|
|HLVd-T92 (Czech Republic)||Approximately 1 fg/µL of RNA transcripts|
|HLVd-X07397||Approximately 1 fg/µL of RNA transcripts|
|1Predicted detection by in silico analysis only.|
For a list of validated hosts, please see the Full Validation Report.