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AmplifyRP® XRT for HLVd (Hop latent viroid)

Part Number:
XCS 76500/0048
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Tech Note: Test Improvement and Protocol Change

AmplifyRP® XRT for HLVd is a rapid RNA amplification and detection platform designed for field-based or laboratory testing of hops and Cannabis spp (including industrial hemp) for Hop latent viroid. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify HLVd RNA at a single operating temperature (42 °C).

AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.

The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.

Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.

Click here for other product documents such as the User Guide or printable barcode.


  • XRT reaction pellets for HLVd
  • Pre-filled 100 µL PD1 Pellet diluent tubes
  • GEB-filled mesh extraction bags
  • User Guide
  • Tech Note

Required but Not Included:


For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: N/A

Full Validation Report

Analytical Sensitivity
Limit of detection: Approximately 1 fg/µL of RNA transcripts
Limit of detection: 1:20,000 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations Analytical Sensitivity
HLVd Michigan isolate (MI, USA) Unknown
HLVd-A2 (China)1 Unknown
HLVd-BoulderHLVd (CO, USA)1 Unknown
HLVd-BR (Brazil)1 Unknown
HLVd-C1 (USA)1 Unknown
HLVd-C2 (USA)1 Unknown
HLVd-CR2 (CA, USA)1 Unknown
HLVd-CR3A (CA, USA)1 Unknown
HLVd-CV_117 (USA)1 Unknown
HLVd-CV_12 (USA) Approximately 100 fg/µL of RNA transcripts
HLVd-CV_38 (USA) Approximately 1 fg/µL of RNA transcripts
HLVd-CV_81 (USA) Approximately 1 fg/µL of RNA transcripts
HLVd-CV_88 (USA) Approximately 10 fg/µL of RNA transcripts
HLVd-CV_93 (USA) Approximately 1 fg/µL of RNA transcripts
HLVd-CV1 (USA)1 Unknown
HLVd-DeltaHLVd (CO, USA)1 Unknown
HLVd-GVdC_HLVd01 (Belgium)1 Unknown
HLVd-H2 (China) Approximately 1 fg/µL of RNA transcripts
HLVd-HpLVd001 (CA, USA)1 Unknown
HLVd-K7 (China)1 Unknown
HLVd-RefSeq (Germany) Approximately 1 fg/µL of RNA transcripts
HLVd-T229 (Czech Republic)1 Unknown
HLVd-T50 (Czech Republic)1 Unknown
HLVd-T92 (Czech Republic) Approximately 1 fg/µL of RNA transcripts
HLVd-X07397 Approximately 1 fg/µL of RNA transcripts
HLVd-Y7 (China)1 Unknown
1Predicted detection by in silico analysis only.

None known

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents