AmplifyRP® XRT for Citrus Greening (HLB)
AmplifyRP® XRT for Citrus Greening (HLB) is a rapid RNA amplification and detection platform designed for testing citrus trees for the three causal agents of citrus greening disease (also known as Huanglongbing or HLB): Candidatus Liberibacter asiaticus (Las), Candidatus Liberibacter americanus (Lam) and Candidatus Liberibacter africanus (Laf). This kit includes lyophilized reaction pellets containing the necessary reagents to amplify target organism DNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for HLB
- Pellet diluent
- 1.5 mL microcentrifuge tubes
- AMP1 extraction buffer
- Sample extraction bags, mesh
- User Guide
Required but Not Included:
- 10 µL Pipette
- 25 µL Pipette
- 1 mL Pipette
- Barrier pipette tips
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT |
Full Validation Report |
Analytical Sensitivity |
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Limit of detection: Approximately 10 ag/µL Laf and Las DNA fragments. Approximately 1 fg/µL Lam RNA D fragments. |
Isolate Names / Geographic Strain Locations | |
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Candidatus Liberibacter africanus (Laf) | Candidatus Liberibacter asiaticus (Las) |
Candidatus Liberibacter americanus (Lam) | Laf-PTSAPSY |
Lam-PW_SP (Brazil) | Lam-Sao Paulo (Brazil) |
Las-A4 (China) | Las-JRPAMB1 (FL, USA) |
Las-JXGC (China) | Las-psy62 |
Las-ReuSP1 (Réunion) | Las-TaiYZ2 (Thailand) |
Las-CoFLP (Columbia) | Las-gxpsy |
Las-Ishi-1 (Japan) |
Cross-reactions | |
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Pathogen Name | |
Candidatus Liberibacter solanacearum (Lso) |
For a list of validated hosts, please see the Full Validation Report.
Additional Documents | ||
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