AmplifyRP® XRT for Foc TR4 (Fusarium oxysporum f.sp. cubense tropical race 4)
AmplifyRP® XRT for Foc TR4 is a rapid DNA amplification and detection platform designed for testing banana root, rhizome, stem, pseudostem, and leaf for Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum), the causal agent of Panama Disease. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Foc TR4 DNA and an exogenous DNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT
|Limit of detection: Approximately 1 fg/µL of total genomic DNA|
|Isolate Names / Geographic Strain Locations|
|Foc TR4 NRRL 36114 (Indonesia)||Foc TR4 NRLL 54006 (MN, USA)|
|Foc TR4 VCG 01213/16|
For a list of validated hosts, please see the Full Validation Report.