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AmplifyRP® XRT for Foc TR4 (Fusarium oxysporum f.sp. cubense tropical race 4)

Part Number:
XCS 48300/0048
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AmplifyRP® XRT for Foc TR4 is a rapid DNA amplification and detection platform designed for testing banana root, rhizome, stem, pseudostem, and leaf for Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum), the causal agent of Panama Disease. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Foc TR4 DNA and an exogenous DNA control at a single operating temperature (42 °C).

AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.

The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.

Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.

Click here for other product documents such as the User Guide or printable barcode.


  • XRT reaction pellets for Foc TR4
  • Pre-filled 100 µL PD1 Pellet diluent tubes
  • AMP1 extraction buffer
  • Sample extraction bags, mesh
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: ROX-labeled control probe (exogenous)

Full Validation Report

Analytical Sensitivity
Limit of detection: Approximately 1 fg/µL of total genomic DNA

Isolate Names / Geographic Strain Locations
Foc TR4 NRRL 36114 (Indonesia) Foc TR4 NRLL 54006 (MN, USA)
Foc TR4 VCG 01213/16

None known

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents