AmplifyRP® XRT for DMV & DCMV (Dahlia mosaic virus and Dahlia common mosaic virus)
AmplifyRP® XRT for DMV & DCMV is a rapid DNA amplification and detection platform designed for testing dahlia plants for Dahlia mosaic virus and Dahlia common mosaic virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify DMV and/or DCMV DNAs and an endogenous DNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
- XRT reaction pellets for DMV & DCMV
- PD1 Pellet diluent
- 1.5 mL microcentrifuge tubes
- GEB-filled mesh extraction bags
- User Guide
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT
|Limit of detection: Approximately 71 copies/reaction of DMV DNA fragments|
|Limit of detection: Approximately 66 copies/reaction of DCMV DNA fragments|
|Isolate Names / Geographic Strain Locations|
|DCMV-BJ (China)||DCMV-Holland (Netherlands)|
|DCMV-NZ (New Zealand)||DCMV-TW (Taiwan)|
|DMV-2 (Netherlands)||DMV-Holland (Netherlands)|
|DMV-RefSeq (OR, USA)||DMV DSMZ PV-1007 (Germany)|
|DMV DSMZ PV-1008 (Germany)|
For a list of validated hosts, please see the Full Validation Report.