AmplifyRP® XRT for BCTV (Beet curly top virus)

Part Number:
XCS 11000/0048
In Stock Sign in to Buy

AmplifyRP® XRT for BCTV is a rapid DNA amplification and detection platform designed for field-based or laboratory testing of Beet curly top virus in many crops including: bean, beet, hemp (Cannabis spp.), pepper, potato, spinach, and tomato. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify BCTV DNA at a single operating temperature (42 °C).

AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.

The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.

Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.

Click here for other product documents such as the User Guide or printable barcode.

Included:

  • XRT reaction pellets for BCTV
  • Pre-filled 100 µL PD1 Pellet diluent tubes
  • GEB-filled mesh extraction bags
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: N/A

Full Validation Report

Analytical Sensitivity
Limit of detection: Approximately 1 fg DNA fragment; 1500 copies/µL

Isolate Names / Geographic Strain Locations Analytical Sensitivity
BCTV-CA (CA, USA) Unknown
BCTV-Can (USA) (Cannabis) Unknown
BCTV-CO (CO, USA) 1:10,000 dilution of infected tissue (pathogen titer unknown)
BCTV-KIM1 (ID, USA) (Kimberly1) Unknown
BCTV-LH71 (CA, USA) (Leafhopper 71) 1 fg / μL DNA fragment; 1800 copies
BCTV-Logan (CA, USA) Unknown
BCTV-Mld (Mexico) (Mild) 10 ag / μL DNA fragment; 20 copies
BCTV-PeCT (NM, USA) (Pepper Curly Top) 1:1,000 dilution of infected tissue (pathogen titer unknown)
BCTV-PeYD2 (NM, USA) (Pepper Yellow Dwarf) Unknown
BCTV-SpCT1 (USA) (Spinach Curly Top) 3 fg / μL DNA fragment; 5500 copies
BCTV-Svr (BCTV-CFH) (Severe) 1:1,000 dilution of infected tissue (pathogen titer unknown)
BCTV-SvrPep (NM, USA) (Severe pepper) 10 ag / μL DNA fragment; 20 copies
BCTV-Wor (Worland) Unknown
1Weaker detection as compared to other isolates
2Predicted detection by in silico analysis only

Cross-reactions Analytical Sensitivity
Spinach severe curly top virus (SSCTV) 10 fg / μL DNA fragment; 18,400 copies

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents