AmplifyRP® XRT for BCTV (Beet curly top virus)
AmplifyRP® XRT for BCTV is a rapid DNA amplification and detection platform designed for field-based or laboratory testing of Beet curly top virus in many crops including: bean, beet, hemp (Cannabis spp.), pepper, potato, spinach, and tomato. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify BCTV DNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT
|Limit of detection: Approximately 1 fg DNA fragment; 1500 copies/µL|
|Isolate Names / Geographic Strain Locations||Analytical Sensitivity|
|BCTV-CA (CA, USA)||Unknown|
|BCTV-Can (USA) (Cannabis)||Unknown|
|BCTV-CO (CO, USA)||1:10,000 dilution of infected tissue (pathogen titer unknown)|
|BCTV-KIM1 (ID, USA) (Kimberly1)||Unknown|
|BCTV-LH71 (CA, USA) (Leafhopper 71)||1 fg / μL DNA fragment; 1800 copies|
|BCTV-Logan (CA, USA)||Unknown|
|BCTV-Mld (Mexico) (Mild)||10 ag / μL DNA fragment; 20 copies|
|BCTV-PeCT (NM, USA) (Pepper Curly Top)||1:1,000 dilution of infected tissue (pathogen titer unknown)|
|BCTV-PeYD2 (NM, USA) (Pepper Yellow Dwarf)||Unknown|
|BCTV-SpCT1 (USA) (Spinach Curly Top)||3 fg / μL DNA fragment; 5500 copies|
|BCTV-Svr (BCTV-CFH) (Severe)||1:1,000 dilution of infected tissue (pathogen titer unknown)|
|BCTV-SvrPep (NM, USA) (Severe pepper)||10 ag / μL DNA fragment; 20 copies|
|1Weaker detection as compared to other isolates
2Predicted detection by in silico analysis only
|Spinach severe curly top virus (SSCTV)||10 fg / μL DNA fragment; 18,400 copies|
For a list of validated hosts, please see the Full Validation Report.