AmplifyRP® XRT for Bcin (Botrytis cinerea)
AmplifyRP® XRT for Bcin is a rapid DNA amplification and detection platform designed for testing grape, hemp (cannabis), rose, and soil for Botrytis cinerea. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Bcin DNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
- XRT reaction pellets for Bcin
- PD1 Pellet diluent
- 1.5 mL microcentrifuge tubes
- AMP1 extraction buffer
- Sample extraction bags, mesh
- User Guide
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: AmplifyRP® XRT
|The assay is 91.7% sensitive between 1 fg/µL and 10 fg/µL. (n=12)
|Limit of detection:
|The assay has a 100% detection rate at 10 fg/µL with DNA fragment. (n=6)
|The assay has a 83.3% detection rate at 1 fg/µL with DNA fragment. (n=6)
|Isolate Names / Geographic Strain Locations
|This assay was designed to detect all strains and isolates of Bcin. One hundred ten distinct samples of Bcin have been experimentally proven to be detected including Botrytis cinerea (asexual stage) and Botrytis fuckeliana (sexual stage).
For a list of validated hosts, please see the Full Validation Report.