ELISA Reagent Set for Tobacco etch virus (TEV)
This ELISA test is a qualitative serological assay for the detection of Tobacco etch virus (TEV) in ornamental and vegetable leaves. TEV is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TEV ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:64,800 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| TEV-HAT (Highly Aphid Transmitted) | TEV-Mex21 (Mexico) |
| TEV-N (USA) | TEV-NAT (Not Aphid Transmitted) |
| TEV PV-69 (WI, USA) | TEV PV-364 |
| TEV-UM (MD, USA) | |
| Cross-reactions | |
|---|---|
| Pepper mottle virus (PepMoV) | Pepper veinal mottle virus (PVMV) |
| Potato virus Y (PVY)1 | Potato virus Y-necrotic strain (PVY-n)2 |
| Tobacco vein mottling virus (TVMV) | |
1Cross-reaction observed with PVY in 2 out of 4 samples with limited data set, including samples of PVY-n.
2Cross-reaction observed with PVY-n in 1 out of 2 samples with limited data set.
For a list of validated hosts, please see the Full Validation Report.
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| Option | Unit | Purchase |
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Reagent Set for Tobacco etch virus (TEV), 96 testwells
SRA 49501/0096
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Shown in cart
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Reagent Set for Tobacco etch virus (TEV), 500 testwells
SRA 49501/0500
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Shown in cart
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Reagent Set for Tobacco etch virus (TEV), 1000 testwells
SRA 49501/1000
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Shown in cart
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Reagent Set for Tobacco etch virus (TEV), 5000 testwells
SRA 49501/5000
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Shown in cart
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