ELISA Reagent Set for Potato virus Y-necrotic strain (PVY-n)

Tech Note: Agdia’s Recommended Method for the Indentification of PVY-n

This ELISA test is a qualitative serological assay for the detection of Potato virus Y - necrotic strain (PVY-n) in potato leaves and sprouts. PVY-n is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles. This test also detects the PVY-NTN strains that cause the tuber necrotic ringspot disease (PTNRD) of potatoes.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PVYn/PVY ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:3,600 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
PVYN-C3 PVYN-LS
PVYN-NC189 (Hungary) PVYN-NC204
PVYN-PEI (Canada) PVYN-Q9
PVYN-T8A PVYN-Tu619 (USA)
PVYN-18 PVYN-19
PVYN-20 PVYN-21
PVYN-25 PVYN-27 (North America)
PVYN-27-92 PVYN-33
PVYN-36 PVYN-37
PVYN-42 PVYN-48
PVYN-54 PVYN-57
PVYN-58 PVYN-605 (Switzerland)
PVYNTN (PTNRD)

Cross-reactions
Potato virus Y-o, strain 141 (PVYO-141) Potato virus Y-o, strain 261 (PVYO-261)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Potato virus Y-necrotic strain (PVY -n), 96 testwells
SRA 26001/0096
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Reagent Set for Potato virus Y-necrotic strain (PVY -n), 500 testwells
SRA 26001/0500
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Reagent Set for Potato virus Y-necrotic strain (PVY -n), 1000 testwells
SRA 26001/1000
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Reagent Set for Potato virus Y-necrotic strain (PVY -n), 5000 testwells
SRA 26001/5000
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