Agdia ImmunoStrip® for Potato virus Y (PVY) and Potato virus Y – necrotic strain (PVY-N)
The Potato virus YN & Potato virus Y (PVYN/PVY) ImmunoStrip® is used to detect the presence of PVY and PVYN in potato leaves and sprouts. PVY is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles. ImmunoStrips® are the perfect screening tool for use in the field, greenhouse, and the lab.
The PVYN/PVY ImmunoStrip® is a multi-line assay that not only identifies the presence of PVY, but also indicates whether the PVY present in a sample is a necrotic strain of the virus. This assay contains two test lines as well as a control line to indicate the test worked properly. One of the two test lines can detect all PVY strains while the second test line detects only PVYN serotype strains. If both the PVY and PVYN test lines are visible, the sample is infected with a PVYN serotype strain. If only the PVY test line is visible, the sample is infected with a PVYO serotype strain. If only the control line is visible, this indicates a negative result for all strains of PVY.
This test is a rapid diagnostic tool that has enhanced value for PVY disease management programs. Potato growers and inspectors can identify PVY in the field and assess whether a plant has a higher risk of developing potato tuber necrosis disease (PTNRD) associated with PVYN recombinant strains.
If you would prefer to add a field-deployable molecular assay to your testing toolkit, please check out Agdia's PVY AmplifyRP® XRT method.
Click here for other product documents such as the User Guide or Validation Report.
Included in ISK Kit:
- ImmunoStrips®
- Sample bags containing SEB4 buffer
- User Guide
Included in STX Item:
- ImmunoStrips®
- User Guide
Required but Not Included:
- Scissors
- Extraction tool
- Agdia tissue homogenizer, marker, or pen
- Letter holder or other device to hold sample extraction bags upright
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: ImmunoStrip® Lateral Flow Device (LFD) |
Full Validation Report |
Analytical Sensitivity |
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Limit of detection (PVYN): 1:1,000 dilution of infected tissue (pathogen titer unknown) |
Limit of detection (PVY): 1:1,000 dilution of infected tissue (pathogen titer unknown) |
Isolate Names / Geographic Strain Locations | |
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PVYN line | PVY line |
Potato virus YN (PVYN) | Potato virus YN (PVYN) |
PVYN-Na-N | Potato virus YO (PVYO) |
PVYN-NE11 | PVYN-Na-N |
PVYN-NTN | PVYN-NE11 |
PVYO-o5 | PVYN-NTN |
PVYO-E | |
PVYO-n:o | |
PVYO-N-Wi | |
PVYO-o5 |
Cross-reactions | |
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None Known |
For a list of validated hosts, please see the Full Validation Report.
Additional Documents | ||
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Option | Unit | Purchase |
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ImmunoStrip for PVYn/PVY, 5 strips and buffer filled bags
ISK 38200/0005
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Shown in cart
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ImmunoStrip for PVYn/PVY, 25 strips and buffer filled bags
ISK 38200/0025
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Shown in cart
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ImmunoStrip for PVYn/PVY ***BUFFER FILLED BAGS NOT INCLUDED***
STX 38200/0025
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Shown in cart
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