ELISA Test Kits for Pseudomonas spp.
This ELISA test is a qualitative serological assay for the detection of Pseudomonas spp. in fruit, ornamental, and vegetable crops. The Pseudomonas spp. ELISA detects Pseudomonas to the genus level and cannot differentiate species. For a full list of species that have experimentally detected, click here.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Pseudomonas spp. ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- RUB6 Enzyme conjugate diluent, 1X Ready to use
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00333)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- RUB6 enzyme conjugate diluent, 1X ready to use
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- RUB6 enzyme conjugate diluent, 1X ready to use
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
|
Test Label: Alkaline Phosphatase |
Full Validation Report (Coming Soon) |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:38,800 dilution of infected tissue (pathogen titer unknown) |
| Species Detected | |
|---|---|
| Pseudomonas aeruginosa | Pseudomonas amygdali pv. lachrymans |
| Pseudomonas amygdali pv. morsprunorum | Pseudomonas cannabina pv. alisalensis |
| Pseudomonas cannabina pv. cannabina | Pseudomonas capsici |
| Pseudomonas cichorii | Pseudomonas corrugata |
| Pseudomonas savastanoi pv. glycinea | Pseudomonas savastanoi pv. phaseolicola |
| Pseudomonas syringae pv. aptata | Pseudomonas syringae pv. atrofaciens |
| Pseudomonas syringae pv. japonica | Pseudomonas syringae pv. pisi |
| Pseudomonas syringae pv. syringae | Pseudomonas syringae pv. tomato |
| Pseudomonas viridiflava | |
| Species Not Detected | |
|---|---|
| Pseudomonas chlororaphis | Pseudomonas fluorescens |
| Pseudomonas lini | Pseudomonas syringae pv. aceris |
| Pseudomonas tremae | |
| Cross-reactions | |
|---|---|
| Stutzerimonas stutzeri | Xanthomonas campestris pv. incanae |
For a list of validated hosts, please see the Full Validation Report (Coming Soon).
| Additional Documents | ||
|---|---|---|
User Guide |
|
|
|
|
|
|
|
|
|
|
|
| Option | Unit | Purchase |
|---|---|---|
|
Pseudomonas spp. Reagent Set, 96 Testwells
SRA 82200/0096
|
Shown in cart
|
|
|
Pseudomonas spp. Reagent Set, 500 Testwells
SRA 82200/0500
|
Shown in cart
|
|
|
Pseudomonas spp. Reagent Set, 1000 Testwells
SRA 82200/1000
|
Shown in cart
|
|
|
Pseudomonas spp. Reagent Set, 5000 Testwells
SRA 82200/5000
|
Shown in cart
|
|
|
Pseudomonas spp. PathoScreen® Complete Kit, 96 Testwells
PSA 82200/0096
|
Shown in cart
|
|
|
Pseudomonas spp. PathoScreen® Complete Kit, 288 Testwells
PSA 82200/0288
|
Shown in cart
|
|
|
Pseudomonas spp. PathoScreen® Complete Kit, 480 Testwells
PSA 82200/0480
|
Shown in cart
|
