ELISA Reagent Set for Plum pox virus (PPV)
This ELISA test is a qualitative serological assay for the detection of Plum pox virus (PPV) in stone fruit leaves. PPV is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PPV ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- Positive control strips
- RUB3 enzyme conjugate diluent
- General extract buffer 4, GEB4 powder1
- TWEEN® 20
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00113)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- Buffer Formulations from Chemicals
- Buffer Instructions from Powders and Concentrates
- Logsheet
1Enough GEB4 sample extraction buffer and TWEEN® 20 is provided in PPV ELISA kits to run 48 samples per plate, in duplicate. Additional GEB4 and TWEEN® 20 should be ordered here if running single samples in single testwells.
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:72,900 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| PPV-An (Marcus ancestor) (Albania) | PPV-C (Cherry) (Moldova) |
| PPV-CR (Cherry Russia) (Russia) | PPV-CV (Cherry Volga) (Russia)4 |
| PPV-D (Dideron) | PPV-EA (El Amar) (Egypt) |
| PPV-M (Marcus) | PPV-Rec (Recombinant) (Serbia) |
| PPV-SC (Sour Cherry) (Russia)1,2 | PPV-T (Turkey) |
| PPV-TAT (Tatarstan) (Russia)1,2 | PPV-Tat-26 (Russia)1,3 |
| PPV-W (Winona) (Canada) | PPV-Y (Yekaterinburg) (Russia)1,2 |
1PPV-SC, PPV-TAT, PPV-Tat-26, and PPV-Y have been externally reported to be detected.
2PPV-SC, PPV-TAT, and PPV-Y have been provisionally suggested as new PPV strains.
3The taxonomic position of PPV-Tat-26 is currently unknown.
4PPV-CV has been externally reported to be detected.
| Cross-reactions | |
|---|---|
| Dasheen mosaic virus (DsMV) | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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| Option | Unit | Purchase |
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Reagent Set for Plum pox virus (PPV), 96 testwells
SRA 31505/0096
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Shown in cart
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Reagent Set for Plum pox virus (PPV), 500 testwells
SRA 31505/0500
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Shown in cart
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Reagent Set for Plum pox virus (PPV), 1000 testwells
SRA 31505/1000
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Shown in cart
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Reagent Set for Plum pox virus (PPV), 5000 testwells
SRA 31505/5000
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Shown in cart
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