ELISA Test Kits for PPV (Plum pox virus)
Tech Note: Product Change
This ELISA test is a qualitative serological assay for the detection of Plum pox virus (PPV) in stone fruit leaves. PPV is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PPV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- Positive control strips
- RUB3 enzyme conjugate diluent
- General extract buffer 4, GEB4 powder1
- TWEEN® 20
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00113)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- Buffer Formulations from Chemicals
- Buffer Instructions from Powders and Concentrates
- Logsheet
1Enough GEB4 sample extraction buffer and TWEEN® 20 is provided in PPV ELISA kits to run 48 samples per plate, in duplicate. Additional GEB4 and TWEEN® 20 should be ordered here if running single samples in single testwells.
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- Positive control strips
- RUB3 enzyme conjugate diluent
- General extract buffer 4, GEB4 powder1
- TWEEN® 20
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- PBST buffer, 20X concentrate, 50 mL
- PathoScreen User Guide
- Tech Note
Required but Not Included:
1Enough GEB4 sample extraction buffer and TWEEN® 20 is provided in PPV ELISA kits to run 48 samples per plate, in duplicate. Additional GEB4 and TWEEN® 20 should be ordered here if running single samples in single testwells.
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- RUB3 enzyme conjugate diluent
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:40,000 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| PPV-An (Marcus ancestor) (Albania) | PPV-C (Cherry) (Moldova) |
| PPV-CR (Cherry Russia) (Russia) | PPV-D (Dideron) |
| PPV-EA (El Amar) (Egypt) | PPV-M (Marcus) |
| PPV-Rec (Recombinant) (Serbia) | PPV-SoC (Sour Cherry) (Moldova) |
| PPV-T (Turkey) | PPV-W (Winona) (Canada) |
| Cross-reactions | |
|---|---|
| Potyvirus alstromeriae (Alstroemeria mosaic virus) (AlMV) | Potyvirus perulycopersici (Peru tomato mosaic virus) (PTV) |
| Potyvirus vetuberosi (Potato virus V) (PVV) | Potyvirus gebatatae (Sweet potato virus G) (SPVG) |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
|---|---|---|
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PPV Reagent Set, 96 Testwells
SRA 31505/0096
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Shown in cart
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PPV Reagent Set, 500 Testwells
SRA 31505/0500
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Shown in cart
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PPV Reagent Set, 1000 Testwells
SRA 31505/1000
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Shown in cart
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PPV Reagent Set, 5000 Testwells
SRA 31505/5000
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Shown in cart
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PPV PathoScreen® Complete Kit, 96 Testwells
PSA 31505/0096
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Shown in cart
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PPV PathoScreen® Complete Kit, 288 Testwells
PSA 31505/0288
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Shown in cart
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PPV PathoScreen® Complete Kit, 480 Testwells
PSA 31505/0480
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Shown in cart
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