ELISA Reagent Set for Plum pox virus (PPV)

This ELISA test is a qualitative serological assay for the detection of Plum pox virus (PPV) in stone fruit leaves. PPV is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PPV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • Positive control strips
  • RUB3 enzyme conjugate diluent
  • General extract buffer 4, GEB4 powder1
  • TWEEN® 20
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

1Enough GEB4 sample extraction buffer and TWEEN® 20 is provided in PPV ELISA kits to run 48 samples per plate, in duplicate. Additional GEB4 and TWEEN® 20 should be ordered here if running single samples in single testwells.

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:72,900 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
PPV-An (Ancestor) PPV-C (Cherry)
PPV-CR (Cherry Russia) PPV-D (Dideron)
PPV-EA (El Amar) PPV-M (Marcus)
PPV-Rec (Recombinant) PPV-T (Turkey)
PPV-W (Winona)

Cross-reactions
Dasheen mosaic virus (DsMV)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Plum pox virus (PPV), 96 testwells
SRA 31505/0096
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Reagent Set for Plum pox virus (PPV), 500 testwells
SRA 31505/0500
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Reagent Set for Plum pox virus (PPV), 1000 testwells
SRA 31505/1000
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Reagent Set for Plum pox virus (PPV), 5000 testwells
SRA 31505/5000
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