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ImmunoStrip® for Plum pox virus (PPV)

The Plum pox virus (PPV) ImmunoStrip® is used to detect the presence of PPV in stone fruit crops. ImmunoStrips® are the perfect screening tool for use in the field, greenhouse, and the lab.

Plum pox virus (Sharka disease) is an aphid transmitted potyvirus that can cause significant loss of yield and marketability of stone fruit (peaches, plum, cherry, etc). PPV is found around the world and is of particular importance in Europe and other Mediterranean regions. PPV detections have been confirmed in the U.S. and Canada in the late 1990's and early 2000's which instigated large scale testing and eradication programs due to the severity of the disease.

A convenient property of the PPV ImmunoStrip® is that it was designed to run in GEB4 extraction buffer; the same extraction buffer used in Agdia's PPV ELISA (USDA approved testing method for PPV) as well as the AmplifyRP® DNA / RNA detection platform. This allows current ELISA users to confirm results by another testing method using the same sample extract.

If you would prefer to add a field-deployable molecular assay to your testing toolkit, please check out Agdia's PPV AmplifyRP® Acceler8® method.

Click here for other product documents such as the User Guide or Validation Report.

Included in ISK Kit:

  • ImmunoStrips®
  • Sample bags containing GEB4 buffer
  • User Guide

Included in STX Item:

Required but Not Included:

  • Scissors
  • Extraction tool
  • Letter holder or other device to hold sample extraction bags upright

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: ImmunoStrip® Lateral Flow Device (LFD)
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:600 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
PPV-An (Marcus ancestor) (Albania) PPV-C (Cherry) (Moldova)
PPV-CR (Cherry Russian) (Russia) PPV-D (Dideron)
PPV-EA (El Amar) (Egypt) PPV-M (Marcus)
PPV-Rec (Recombinant) (Serbia) PPV-T (Turkey)
PPV-W (Winona) (Canada)

None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
ImmunoStrip® for Plum pox virus (PPV), 5 strips & buffer filled bags
ISK 31505/0005
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ImmunoStrip® for Plum pox virus (PPV), 25 strips & buffer filled bags
ISK 31505/0025
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ImmunoStrips® only for Plum pox virus (PPV) ***BUFFER FILLED BAGS NOT INCLUDED***
STX 31505/0025
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