AmplifyRP® XRT for HSVd (Hop stunt viroid)
Tech Note: Protocol Change
AmplifyRP® XRT for HSVd is a rapid RNA amplification and detection platform designed for testing of citrus, hemp, hop, and grape for Hop stunt viroid. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify HSVd RNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for HSVd
- PD1 Pellet diluent
- 1.5 mL microcentrifuge tubes
- GEB-filled mesh extraction bags
- User Guide
- Tech Note
Required but Not Included:
- 25 µL Pipette
- 1 mL Pipette
- 25 µL and 1mL Barrier Pipette Tips
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
|
Test Format: AmplifyRP® XRT |
Full Validation Report |
| Analytical Sensitivity | |
|---|---|
| Analytical Sensitivity: | The assay is 92.5% sensitive between 1 pg/µL and 100 fg/µL. (n=40) |
| Limit of detection: | The assay has a 100% detection rate at 1 pg/µL with RNA transcripts. (n=20) |
| The assay has a 85.0% detection rate at 100 fg/µL with RNA transcripts. (n=20) | |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| HSVd citrus-type | HSVd hop-type |
| HSVd plum-type | HSVd-CAZ2 (Mexico) |
| HSVd-CC-H | HSVd-cl2 |
| HSVd-E77_HSVd (Spain) | HSVd-HSVd.apr20 (Cyprus) |
| HSVd-HSVd-maz | HSVd-HSVd-Mor-ValL_Big |
| HSVd-HT2 (Iran) | HSVd-PM-26x |
| HSVd-SC-18 (China) | HSVd-tk4 (Turkey) |
| Cross-reactions | |
|---|---|
| None known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
|---|---|---|
|
|
|
|
|
|
|
|
||