Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Intended Use:

This ELISA test is a qualitative serological screening tool validated to detect Lettuce mosaic virus (LMV) in lettuce leaf, seedling, and seed. The test is specific, sensitive and robust. All samples can be ground in the same sample buffer at a sample to buffer ratio of 1:10 (g/ml). No cross-reactivity with other potyviruses or other lettuce viruses has been found. The test consistently was able to detect LMV infected seed lots using 500 seed subsamples.

The robustness of the assay offers the user greater flexibility in performing the test. Optimal results are achieved when following the Agdia protocol, however, excellent results are also achieved using other protocols. The Agdia LMV ELISA is completely compatible with the International Seed Federation (ISF) reference method.

Cross-reactions: None

Includes:

• Capture antibody
• Alkaline phosphatase conjugated antibodies
• 96-well microtiter plates, solid* (*strip in single plate set)
• User Guide
• Tech Note
• Buffer Formulations
• Logsheet