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ELISA Reagent Set for Lettuce mosaic virus (LMV)

Tech Note: Alternate Seed Protocol

This ELISA test is a qualitative serological assay for the detection of Lettuce mosaic virus (LMV) in lettuce leaf, seedling, and seed. LMV is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.

The robustness of the assay offers the user greater flexibility in performing the test. Optimal results are achieved when following the Agdia protocol, however, excellent results are also achieved using other protocols. The Agdia LMV ELISA is completely compatible with the International Seed Federation (ISF) reference method. The test consistently was able to detect LMV infected seed lots using 500 seed subsamples.

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:4,000 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of LMV. Nine distinct samples of LMV have been experimentally proven to be detected.

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Lettuce mosaic virus (LMV), 96 testwells
SRA 45801/0096
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Reagent Set for Lettuce mosaic virus (LMV), 500 testwells
SRA 45801/0500
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Reagent Set for Lettuce mosaic virus (LMV), 1000 testwells
SRA 45801/1000
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Reagent Set for Lettuce mosaic virus (LMV), 5000 testwells
SRA 45801/5000
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