ELISA Test Kits for PVY (Potato virus Y)
Tech Note: PVY ELISA Protocol Change and Product Improvement
This ELISA test is a qualitative serological assay for the detection of Potato virus Y (PVY) in potato leaf, sprouts, and tubers as well as other solanaceous crops. PVY is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PVY ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- ECI buffer, 5X concentrate
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
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Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:10,000 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| PVYNE-11 | PVY-Alt (PVYN:O) |
| PVY-HI-14 (PVYC) | PVY-HR1 (PVYZ) (PVYNTN) |
| PVY-ID269 (PVYO-O5) | PVY-Mont (PVYN) |
| PVY-N1 (PVYN-Wi) | PVY-Oz (PVYO) |
| PVY-Poha6 (PVYC) (PVYC-Poha) | PVY-Pondo4 (PVY261-4) (PVYO) |
| PVY-PVY-AGA (PVYE) (PVYN/AST) | PVY-Tam17 (SA-N) (N serotype)1 |
| Isolate Names / Geographic Strain Locations Not Detected | |
|---|---|
| PVY-Tam15 (SA-N) (no serotype)1 | |
1Isolate unable to systemically spread in potato (Green et. al.)
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
|---|---|---|
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PVY Reagent Set, 96 Testwells
SRA 20001/0096
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Shown in cart
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PVY Reagent Set, 500 Testwells
SRA 20001/0500
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Shown in cart
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PVY Reagent Set, 1000 Testwells
SRA 20001/1000
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Shown in cart
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PVY Reagent Set, 5000 Testwells
SRA 20001/5000
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Shown in cart
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PVY PathoScreen® Complete Kit, 96 Testwells
PSA 20001/0096
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Shown in cart
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PVY PathoScreen® Complete Kit, 288 Testwells
PSA 20001/0288
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Shown in cart
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PVY PathoScreen® Complete Kit, 480 Testwells
PSA 20001/0480
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Shown in cart
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