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PathoScreen® Kit for Zucchini yellow mosaic virus (ZYMV)

This ELISA test is a qualitative serological assay for the detection of Zucchini yellow mosaic virus (ZYMV) in cucurbit leaves. ZYMV is a member of the Potyvirus genus known for their non-enveloped, flexuous, filamentous virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ZYMV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:97,200 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ZYMV-1318 (France) ZYMV-BS1 (South Korea)1
ZYMV-CA (CA, USA) ZYMV-CG-04-65 (France)
ZYMV-CH-98-60 (China) ZYMV-CH-98-77 (China)
ZYMV-CT (CT, USA) ZYMV-E9 (France)
ZYMV-IT (Italy) ZYMV-MAU (Mauritius)
ZYMV-MAY (Mayotte) ZYMV-MT7 (Martinique Island)
ZYMV-R5A (Reunion Island) ZYMV-SIN (Singapore)

1ZYMV-BS1 has been externally reported to be detected.

None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Zucchini yellow mosaic virus (ZYMV), 96 testwells
PSA 77700/0096
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PathoScreen® Kit, Zucchini yellow mosaic virus (ZYMV), 288 testwells
PSA 77700/0288
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PathoScreen® Kit, Zucchini yellow mosaic virus (ZYMV), 480 testwells
PSA 77700/0480
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