PathoScreen® Kit for Xylella fastidiosa (Xf)

This ELISA product is a qualitative assay for the detection of Xylella fastidiosa (Xf), the causal agent of bacterial leaf scorch, Pierce's disease, and phony peach disease. This pathogen has a wide host range, including almond, blackberry, citrus, grapevine, and olive.

The antibodies for this assay were screened against multiple isolates of three subspecies within the X. fastidiosa species, detecting all true positives, including those from infected Oleander. Exclusivity panels revealed no cross-reactivity with multiple common bacterial, fungal and viral pathogens. Moreover, no host-reaction was observed with healthy tissue from any common host of X. fastidiosa we tested.

Xylella fastidiosa spreads throughout the plant via the xylem vessels and can be distributed differentially within the host. Due to this localization, we recommend testing petiole or stem samples from multiple locations on symptomatic plants. Please note that continuing external research suggests root tissue provides the most consistent accurate results when testing peach for the presence of X. fastidiosa.

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • RUB6, 1X Ready to use
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 2x106 CFU/mL

Isolate Names / Geographic Strain Locations
Xylella fastidiosa subsp. fastidiosa Xylella fastidiosa subsp. multiplex
Xylella fastidiosa subsp. sandyi Xylella fastidiosa Almond isolate
Xylella fastidiosa Blueberry isolate Xylella fastidiosa Elm isolate
Xylella fastidiosa Grape isolate Xylella fastidiosa Oak isolate
Xylella fastidiosa Plum isolate Xylella fastidiosa Temecula isolate

Cross-reactions
Pseudomonas syringae pv. syringae Xanthomonas arboricola pv. pruni

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Xylella fastidiosa (Xf), 96 testwells
PSA 34503/0096
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PathoScreen® Kit, Xylella fastidiosa (Xf), 288 testwells
PSA 34503/0288
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PathoScreen® Kit, Xylella fastidiosa (Xf), 480 testwells
PSA 34503/0480
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