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PathoScreen® Kit for Tobacco streak virus (TSV)

This ELISA test is a qualitative serological assay for the detection of Tobacco streak virus (TSV) in ornamental and vegetable leaves. TSV is a member of the Ilarvirus genus known for their non-enveloped, quasi-spherical-shaped virus particles. The test demonstrates cross-reactivity with Strawberry necrotic shock virus (SNSV), therefore, can also be used as a detection tool for SNSV.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TSV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:8,100 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
TSV-B (ATCC® PV-353™) (Brazil) TSV-GV (ATCC® PV-352™) (NY, USA)
TSV-NC (ATCC® PV-351™) (NC, USA) TSV-R (Strawberry necrotic shock virus (SNSV))
TSV-U135D TSV-WC (ATCC® PV-276™) (WI, USA)

Strawberry necrotic shock virus (SNSV)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tobacco streak virus (TSV), 96 testwells
PSA 25500/0096
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PathoScreen® Kit, Tobacco streak virus (TSV), 288 testwells
PSA 25500/0288
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PathoScreen® Kit, Tobacco streak virus (TSV), 480 testwells
PSA 25500/0480
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