ELISA Test Kits for TSV (Tobacco streak virus)
This ELISA test is a qualitative serological assay for the detection of Tobacco streak virus (TSV) in ornamental and vegetable leaves. TSV is a member of the Ilarvirus genus known for their non-enveloped, quasi-spherical-shaped virus particles. The test demonstrates cross-reactivity with Strawberry necrotic shock virus (SNSV), therefore, can also be used as a detection tool for SNSV.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TSV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:8,100 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| TSV-B (ATCC® PV-353™) (Brazil) | TSV-GV (ATCC® PV-352™) (NY, USA) |
| TSV-NC (ATCC® PV-351™) (NC, USA) | TSV-R (Strawberry necrotic shock virus (SNSV)) |
| TSV-Rh (China)1 | TSV-U135D |
| TSV-WC (ATCC® PV-276™) (WI, USA) | |
1TSV-Rh has been externally reported to be detected.
| Cross-reactions | |
|---|---|
| Strawberry necrotic shock virus (SNSV) | |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
|---|---|---|
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TSV Reagent Set, 96 Testwells
SRA 25500/0096
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Shown in cart
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TSV Reagent Set, 500 Testwells
SRA 25500/0500
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Shown in cart
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TSV Reagent Set, 1000 Testwells
SRA 25500/1000
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Shown in cart
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TSV Reagent Set, 5000 Testwells
SRA 25500/5000
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Shown in cart
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TSV PathoScreen® Complete Kit, 96 Testwells
PSA 25500/0096
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Shown in cart
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TSV PathoScreen® Complete Kit, 288 Testwells
PSA 25500/0288
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Shown in cart
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TSV PathoScreen® Complete Kit, 480 Testwells
PSA 25500/0480
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Shown in cart
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