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PathoScreen® Kit for Tobacco ringspot virus (TRSV)

This ELISA test is a qualitative serological assay for the detection of Tobacco ringspot virus (TRSV) in ornamental and vegetable tissues. TRSV is a member of the Nepovirus genus known for their transmission by nematodes.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TRSV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:194,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
TRSV PV-48 (Eucharis mottle) (Peru) TRSV PV-97 (Steere's isolate)
TRSV PV-98 (WS-1) (Maryland) TRSV PV-125 (Watermelon)
TRSV PV-157 (Riesling Grape) (New York) TRSV PV-172 (Rubus) (North Carolina)
TRSV Potato calico isolate

Potato black ringspot virus (PBRSV)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tobacco ringspot virus (TRSV), 96 testwells
PSA 64001/0096
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PathoScreen® Kit, Tobacco ringspot virus (TRSV), 288 testwells
PSA 64001/0288
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PathoScreen® Kit, Tobacco ringspot virus (TRSV), 480 testwells
PSA 64001/0480
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