PathoScreen® Kit for Tomato ringspot virus (ToRSV)

This ELISA test is a qualitative serological assay for the detection of Tomato ringspot virus (ToRSV) in ornamental and vegetable leaves. ToRSV is a member of the Nepovirus genus known for their transmission by nematodes.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ToRSV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • RUB6 enzyme conjugate diluent, 1X ready to use
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer 3, GEB3
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:10,800 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ToRSV-Ch (Chickadee) ToRSV PV-0049 (Geranium) (Denmark)
ToRSV PV-0380 (Apple) (PA, USA) ToRSV PV-0381 (Grape) (NY, USA)
ToRSV-PYBMV (Peach)

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tomato ringspot virus (ToRSV), 96 testwells
PSA 22001/0096
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PathoScreen® Kit, Tomato ringspot virus (ToRSV), 288 testwells
PSA 22001/0288
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PathoScreen® Kit, Tomato ringspot virus (ToRSV), 480 testwells
PSA 22001/0480
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