PathoScreen® Kit for Tobacco mosaic virus (TMV)

Tech Note: Test Format and Protocol Change

This ELISA test is a qualitative serological assay for the detection of Tobacco mosaic virus (TMV), the causal agent of Tobacco mosaic disease, in crops including vegetables, fruits, and ornamentals. TMV is a member of the Tobamovirus genus known for their rod-shaped virus particles. This ELISA has a wide cross-reactivity with other Tobamoviruses. For a complete list click here.

Tobacco mosaic virus is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TMV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Synthetic Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 100-1000 pg/mL of purified virus

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of TMV. Nine distinct samples of TMV have been experimentally proven to be detected.

Cross-reactions Analytical Sensitivity
Bell pepper mottle virus (BPeMV) 1:50,000 dilution of infected tissue (pathogen titer unknown)
Cucumber green mottle mosaic virus (CGMMV) 10-1000 ng/mL of purified virus
Kyuri green mottle mosaic virus (KGMMV) 1:500,000 to 1:1,000,000 dilution of infected tissue (pathogen titer unknown)
Odontoglossum ringspot virus (ORSV) 1:100,000 dilution of infected tissue (pathogen titer unknown)
Pepper mild mottle virus (PMMoV) 10-100 ng/mL of purified virus
Piper chlorosis virus (PChV)1,2 Unknown
Ribgrass mosaic virus (RMV) 1:5,000,000 dilution of infected tissue (pathogen titer unknown)
Sunn-hemp mosaic virus (SHMV) 1:100,000 to 1:500,000 dilution of infected tissue (pathogen titer unknown)
Tobacco mild green mosaic virus (TMGMV) 1:500,000 dilution of infected tissue (pathogen titer unknown)
Tomato brown rugose fruit virus (ToBRFV) 10-100 pg/mL of purified virus
Tomato mosaic virus (ToMV) 1-10 ng/mL of purified virus
Tomato mottle mosaic virus (ToMMV) 1:50,000 to 1:100,000 dilution of infected tissue (pathogen titer unknown)
Turnip vein clearing virus (TVCV) 1:5,000,000 dilution of infected tissue (pathogen titer unknown)
1Confirmed experimentally at Agdia, Inc.
2Reported to detect Piper chlorosis virus (PChV), a possible novel Tobamovirus.

Despite the broad reactivity of this test, it is recommended that specific viral tests be used when available as they are optimized for test specificity and sensitivity which may exceed the sensitivity and specificity of the TMV assay to other Tobamoviruses. If you have confirmed the detection of a likely cross-reaction Tobamovirus or any Tobamoviruses not on this list, please contact us.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tobacco mosaic virus (TMV), 96 testwells
PSA 57400/0096
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PathoScreen® Kit, Tobacco mosaic virus (TMV), 288 testwells
PSA 57400/0288
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PathoScreen® Kit, Tobacco mosaic virus (TMV), 480 testwells
PSA 57400/0480
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