Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Intended Use:

This ELISA test is a qualitative seriological assay for detection of Tobacco mosaic virus (TMV) in crops such as vegetable, fruit, and ornamentals. Tobacco mosaic virus is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.

This test detects a variety of viruses from the tobamovirus group including Tobacco mosaic virus-common strain (TMV-c), Tomato mosaic virus (ToMV), and Sunn hemp mosaic virus (SHMV). This broadly reacting TMV assay is recommended whenever you wish to test for the presence of TMV without needing to distinguish TMV-c from other tobamoviruses. This test, however, is not a tobamovirus group test. For example, it does not react with Ribgrass mosaic virus.

Includes:

• Antibody-coated 96-well microtiter plates
• Alkaline phosphatase enzyme conjugate
• ECI buffer, 5X concentrate
• PNP substrate tablets, 5 mg each
• PNP substrate buffer, 5X concentrate
• Positive control
• PBST buffer, 20X concentrate, 50 ml
• Tween-20
• General extract buffer, GEB
• User Guide
• Tech Note