ELISA Test Kits for TMV (Tobacco mosaic virus)
This ELISA test is a qualitative serological assay for the detection of Tobacco mosaic virus (TMV), the causal agent of Tobacco mosaic disease, in crops including vegetables, fruits, and ornamentals. TMV is a member of the Tobamovirus genus known for their rod-shaped virus particles. This ELISA has a wide cross-reactivity with other Tobamoviruses. For a complete list click here.
Tobacco mosaic virus is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TMV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Synthetic Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
|
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 100-1000 pg/mL of purified virus |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| This assay was designed to detect all strains and isolates of TMV. Thirty-six distinct samples of TMV have been experimentally proven to be detected. | |
| Cross-reactions: (Virus Name) (Species Name) |
Analytical Sensitivity |
|---|---|
| African eggplant-associated virus (AEaV)2 Proposed Tobamovirus |
Unknown |
| Bell pepper mottle virus (BPeMV) Tobamovirus maculacapsici |
1:50,000 dilution of infected tissue (pathogen titer unknown) |
| Brugmansia latent virus (BrLV)4> Proposed Tobamovirus |
Unknown |
| Chili pepper mild mottle virus (CPMMoV)5 Proposed Tobamovirus |
Unknown |
| Cucumber green mottle mosaic virus (CGMMV) Tobamovirus viridimaculae |
10-1000 ng/mL of purified virus |
| Cucumber mottle virus (CMoV) Tobamovirus cucumeris |
Unknown |
| Frangipani mosaic virus (FrMV) Tobamovirus frangipani |
Unknown |
| Kyuri green mottle mosaic virus (KGMMV) Tobamovirus kyuri |
1:500,000 to 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| Obuda pepper virus (ObPV) Tobamovirus obudae |
Unknown |
| Odontoglossum ringspot virus (ORSV) Tobamovirus odontoglossi |
1:100,000 dilution of infected tissue (pathogen titer unknown) |
| Paprika mild mottle virus (PaMMV) Tobamovirus paprikae |
Unknown |
| Pepper mild mottle virus (PMMoV) Tobamovirus capsici |
10-100 ng/mL of purified virus |
| Piper chlorosis virus (PChV)1 Proposed Tobamovirus |
Unknown |
| Rehmannia mosaic virus (ReMV) Tobamovirus rehmanniae |
Unknown |
| Ribgrass mosaic virus (RMV) Tobamovirus plantagonis |
1:5,000,000 dilution of infected tissue (pathogen titer unknown) |
| Scopolia mild mottle virus (SMMoV)6 Proposed Tobamovirus |
Unknown |
| Streptocarpus flower break virus (SFBV) Tobamovirus streptocarpi |
Unknown |
| Sunn-hemp mosaic virus (SHMV) Tobamovirus crotalariae |
1:100,000 to 1:500,000 dilution of infected tissue (pathogen titer unknown) |
| Tobacco mild green mosaic virus (TMGMV) Tobamovirus mititessellati |
1:500,000 dilution of infected tissue (pathogen titer unknown) |
| Tomato brown rugose fruit virus (ToBRFV) Tobamovirus fructirugosum |
10-100 pg/mL of purified virus |
| Tomato mosaic virus (ToMV) Tobamovirus tomatotessellati |
1-10 ng/mL of purified virus |
| Tomato mottle mosaic virus (ToMMV) Tobamovirus maculatessellati |
1:50,000 to 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| Turnip vein-clearing virus (TVCV) Tobamovirus rapae |
1:5,000,000 dilution of infected tissue (pathogen titer unknown) |
| Wasabi mottle virus (WMoV) Tobamovirus wasabi |
Unknown |
| Youcai mosaic virus (YoMV) Tobamovirus youcai |
Unknown |
| Zucchini green mottle mosaic virus (ZGMMV)3 Tobamovirus cucurbitae |
Unknown |
1Piper chlorosis virus (PChV) has been reported to be a possible
novel Tobamovirus.
2African eggplant-associated virus (AEaV) has been reported to be a possible novel Tobamovirus.
31 out of 2 isolates of Zucchini green mottle mosaic virus (ZGMMV) were detected.
4Brugmansia latent virus (BrLV) has been reported to be a possible novel Tobamovirus.
5Chili pepper mild mottle virus (CPMMoV) has been reported to be a possible novel Tobamovirus.
6Scopolia mild mottle virus (SMMoV) has been reported to
be a possible novel Tobamovirus.
Despite the broad reactivity of this test, it is recommended that specific viral tests be used when available as they are optimized for test specificity and sensitivity which may exceed the sensitivity and specificity of the TMV assay to other Tobamoviruses. If you have confirmed the detection of a likely cross-reaction Tobamovirus or any Tobamoviruses not on this list, please contact us.
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
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TMV Reagent Set, 96 Testwells
SRA 57400/0096
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Shown in cart
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TMV Reagent Set, 500 Testwells
SRA 57400/0500
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Shown in cart
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TMV Reagent Set, 1000 Testwells
SRA 57400/1000
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Shown in cart
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TMV Reagent Set, 5000 Testwells
SRA 57400/5000
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Shown in cart
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TMV PathoScreen® Complete Kit, 96 Testwells
PSA 57400/0096
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Shown in cart
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TMV PathoScreen® Complete Kit, 288 Testwells
PSA 57400/0288
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Shown in cart
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TMV PathoScreen® Complete Kit, 480 Testwells
PSA 57400/0480
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Shown in cart
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