ELISA Test Kits for TMV (Tobacco mosaic virus)
Tech Note: Test Format and Protocol Change
This ELISA test is a qualitative serological assay for the detection of Tobacco mosaic virus (TMV), the causal agent of Tobacco mosaic disease, in crops including vegetables, fruits, and ornamentals. TMV is a member of the Tobamovirus genus known for their rod-shaped virus particles. This ELISA has a wide cross-reactivity with other Tobamoviruses. For a complete list click here.
Tobacco mosaic virus is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TMV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
Choose from the following formats (see the Included Items tab for full details):
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Synthetic Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
- Tech Note
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 100-1000 pg/mL of purified virus |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| This assay was designed to detect all strains and isolates of TMV. Nine distinct samples of TMV have been experimentally proven to be detected. | |
| Cross-reactions | Analytical Sensitivity |
|---|---|
| Bell pepper mottle virus (BPeMV) | 1:50,000 dilution of infected tissue (pathogen titer unknown) |
| Chili pepper mild mottle virus (CPMMoV) | Unknown |
| Cucumber green mottle mosaic virus (CGMMV) | 10-1000 ng/mL of purified virus |
| Frangipani mosaic virus (FrMV) | Unknown |
| Kyuri green mottle mosaic virus (KGMMV) | 1:500,000 to 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| Odontoglossum ringspot virus (ORSV) | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| Pepper mild mottle virus (PMMoV) | 10-100 ng/mL of purified virus |
| Piper chlorosis virus (PChV)1,2 | Unknown |
| Ribgrass mosaic virus (RMV) | 1:5,000,000 dilution of infected tissue (pathogen titer unknown) |
| Sunn-hemp mosaic virus (SHMV) | 1:100,000 to 1:500,000 dilution of infected tissue (pathogen titer unknown) |
| Tobacco mild green mosaic virus (TMGMV) | 1:500,000 dilution of infected tissue (pathogen titer unknown) |
| Tomato brown rugose fruit virus (ToBRFV) | 10-100 pg/mL of purified virus |
| Tomato mosaic virus (ToMV) | 1-10 ng/mL of purified virus |
| Tomato mottle mosaic virus (ToMMV) | 1:50,000 to 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| Turnip vein clearing virus (TVCV) | 1:5,000,000 dilution of infected tissue (pathogen titer unknown) |
| 1Confirmed experimentally at Agdia, Inc. | |
| 2Reported to detect Piper chlorosis virus (PChV), a possible novel Tobamovirus. | |
Despite the broad reactivity of this test, it is recommended that specific viral tests be used when available as they are optimized for test specificity and sensitivity which may exceed the sensitivity and specificity of the TMV assay to other Tobamoviruses. If you have confirmed the detection of a likely cross-reaction Tobamovirus or any Tobamoviruses not on this list, please contact us.
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
|---|---|---|
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TMV Reagent Set, 96 Testwells
SRA 57400/0096
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Shown in cart
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TMV Reagent Set, 500 Testwells
SRA 57400/0500
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Shown in cart
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TMV Reagent Set, 1000 Testwells
SRA 57400/1000
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Shown in cart
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TMV Reagent Set, 5000 Testwells
SRA 57400/5000
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Shown in cart
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TMV PathoScreen® Complete Kit, 96 Testwells
PSA 57400/0096
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Shown in cart
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TMV PathoScreen® Complete Kit, 288 Testwells
PSA 57400/0288
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Shown in cart
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TMV PathoScreen® Complete Kit, 480 Testwells
PSA 57400/0480
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Shown in cart
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