PathoScreen® Kit for Squash mosaic virus (SqMV)

Tech Note: Alternate Seed Protocol

This ELISA test is a qualitative serological assay for the detection of Squash mosaic virus (SqMV) in cucurbit leaf and seed. SqMV is a member of the Comovirus genus known for their non-enveloped, icosahedral-shaped virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's SqMV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:14,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of SqMV. Ninety seven distinct samples of SqMV have been experimentally proven to be detected.

None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Squash mosaic virus (SqMV), 96 testwells
PSA 26400/0096
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PathoScreen® Kit, Squash mosaic virus (SqMV), 288 testwells
PSA 26400/0288
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PathoScreen® Kit, Squash mosaic virus (SqMV), 480 testwells
PSA 26400/0480
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