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PathoScreen® Kit for Squash mosaic virus (SqMV)

Tech Note: Alternate Seed Protocol

This ELISA test is a qualitative serological assay for the detection of Squash mosaic virus (SqMV) in cucurbit leaf and seed. SqMV is a member of the Comovirus genus known for their non-enveloped, icosahedral-shaped virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's SqMV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:14,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of SqMV. Ninety-seven distinct samples of SqMV have been experimentally proven to be detected.

None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Squash mosaic virus (SqMV), 96 testwells
PSA 26400/0096
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PathoScreen® Kit, Squash mosaic virus (SqMV), 288 testwells
PSA 26400/0288
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PathoScreen® Kit, Squash mosaic virus (SqMV), 480 testwells
PSA 26400/0480
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