PathoScreen® Kit for Ribgrass mosaic virus (RMV)

This ELISA test is a qualitative serological assay for the detection of Ribgrass mosaic virus (RMV) in crops including vegetables and ornamentals. RMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.

Ribgrass mosaic virus is classified as a subgroup 3 Tobamovirus, and this subgroup includes Turnip vein clearing virus (TVCV) and Youcai mosaic virus (YoMV). This classification was designated based on common host range and serological cross-reactivity due to amino acid composition of coat proteins. Ribgrass mosaic virus has an ever-expanding host range that includes multiple plant families containing numerous species of ornamental and horticultural crops.

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  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:291,600 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of RMV. Thirty-one distinct samples of RMV have been experimentally proven to be detected.

Piper chlorosis virus (PChV)1,2 Tobacco mosaic virus (TMV)
Tomato mosaic virus (ToMV) Turnip vein-clearing virus (TVCV)
1Confirmed experimentally at Agdia, Inc.
2Reported to detect Piper chlorosis virus (PChV), a possible novel Tobamovirus.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Ribgrass mosaic virus (RMV), 96 testwells
PSA 57601/0096
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PathoScreen® Kit, Ribgrass mosaic virus (RMV), 288 testwells
PSA 57601/0288
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PathoScreen® Kit, Ribgrass mosaic virus (RMV), 480 testwells
PSA 57601/0480
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