PathoScreen® Kit for Ribgrass mosaic virus (RMV)

This ELISA test is a qualitative serological assay for the detection of Ribgrass mosaic virus (RMV) in crops including vegetables and ornamentals. RMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.

Ribgrass mosaic virus is classified as a subgroup 3 Tobamovirus, and this subgroup includes Turnip vein clearing virus (TVCV) and Youcai mosaic virus (YoMV). This classification was designated based on common host range and serological cross-reactivity due to amino acid composition of coat proteins. Ribgrass mosaic virus has an ever-expanding host range that includes multiple plant families containing numerous species of ornamental and horticultural crops.

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:291,600 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of RMV. Thirty-one distinct samples of RMV have been experimentally proven to be detected.

Cross-reactions
Tobacco mosaic virus (TMV) Tomato mosaic virus (ToMV)
Turnip vein-clearing virus (TVCV)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Ribgrass mosaic virus (RMV), 96 testwells
PSA 57601/0096
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PathoScreen® Kit, Ribgrass mosaic virus (RMV), 288 testwells
PSA 57601/0288
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PathoScreen® Kit, Ribgrass mosaic virus (RMV), 480 testwells
PSA 57601/0480
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