ELISA Test Kits for RMV, TVCV, WMoV & YoMV
Tech Note: Name Change
This ELISA test is a qualitative serological assay for the detection of Ribgrass mosaic virus (RMV), Turnip vein-clearing virus (TVCV), Wasabi mottle virus (WMoV), and Youcai mosaic virus (YoMV) in crops including vegetables and ornamentals. This test will detect RMV, TVCV, WMoV, and YoMV but will not differentiate between them. RMV, TVCV, WMoV, and YoMV are members of the Tobamovirus genus known for their rod-shaped virus particles.
RMV, TVCV, WMoV, and YoMV are classified as a subgroup 3 Tobamovirus. This classification was designated based on common host range and serological cross-reactivity due to amino acid composition of coat proteins. This subgroup has an ever-expanding host range that includes multiple plant families containing numerous species of ornamental and horticultural crops.
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
- Tech Note
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
|
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:291,600 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | ||
|---|---|---|
| RMV-PV-0145 | RMV-Plantago (ATCC® PV-229™) (KS, USA) | |
| TVCV-HR (ATCC® PV-46™) (NJ, USA) | TVCV-PV-0147 (Yugoslavia) | |
| TVCV-PV-0148 | TVCV-PV-0361 (USA) | |
| TVCV-PV-1341 (Germany) | TVCV-PV-1366 (Germany) | |
| WMoV-Tochigi 1 (Japan) | YoMV-722 (Japan) | |
| YoMV-C (Japan) | YoMV-NA23 (Japan) | |
| YoMV-PV-0527 (Germany) | YoMV-YoM-Toy2 (Japan) | |
| YoMV-YoM-Toy5a (Japan) | ||
| Cross-reactions | |
|---|---|
| Virus Name | Species Name |
| Brugmansia latent virus (BrLV)2 | N/A |
| Cucumber mottle virus (CMoV) | Tobamovirus cucumeris |
| Paprika mild mottle virus (PaMMV) | Tobamovirus paprikae |
| Piper chlorosis virus (PChV)1 | N/A |
| Rehmannia mosaic virus (ReMV) | Tobamovirus rehmanniae |
| Streptocarpus flower break virus (SFBV) | Tobamovirus streptocarpi |
| Tobacco mosaic virus (TMV) | Tobamovirus tabaci |
| Tomato mosaic virus (ToMV) | Tobamovirus tomatotessellati |
1Piper chlorosis virus (PChV) has been reported to be a possible
novel Tobamovirus.
2Brugmansia latent virus (BrLV) has been reported to be a possible novel Tobamovirus.
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
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RMV/TVCV/WMoV/YoMV Reagent Set, 96 Testwells
SRA 57601/0096
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Shown in cart
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RMV/TVCV/WMoV/YoMV Reagent Set, 500 Testwells
SRA 57601/0500
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Shown in cart
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RMV/TVCV/WMoV/YoMV Reagent Set, 1000 Testwells
SRA 57601/1000
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Shown in cart
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RMV/TVCV/WMoV/YoMV Reagent Set, 5000 Testwells
SRA 57601/5000
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Shown in cart
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RMV/TVCV/WMoV/YoMV PathoScreen® Complete Kit, 96 Testwells
PSA 57601/0096
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Shown in cart
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RMV/TVCV/WMoV/YoMV PathoScreen® Complete Kit, 288 Testwells
PSA 57601/0288
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Shown in cart
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RMV/TVCV/WMoV/YoMV PathoScreen® Complete Kit, 480 Testwells
PSA 57601/0480
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Shown in cart
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