ELISA Reagent Set for Ribgrass mosaic virus (RMV)
This ELISA test is a qualitative serological assay for the detection of Ribgrass mosaic virus (RMV) in crops including vegetables and ornamentals. RMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.
Ribgrass mosaic virus is classified as a subgroup 3 Tobamovirus, and this subgroup includes Turnip vein clearing virus (TVCV) and Youcai mosaic virus (YoMV). This classification was designated based on common host range and serological cross-reactivity due to amino acid composition of coat proteins. Ribgrass mosaic virus has an ever-expanding host range that includes multiple plant families containing numerous species of ornamental and horticultural crops.
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
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| Limit of detection: 1:291,600 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | ||
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| This assay was designed to detect all strains and isolates of RMV. Thirty-one distinct samples of RMV have been experimentally proven to be detected. |
| Cross-reactions | |
|---|---|
| Chili pepper mild mottle virus (CPMMoV)1 | Piper chlorosis virus (PChV)2,3 |
| Tobacco mosaic virus (TMV) | Tomato mosaic virus (ToMV) |
| Turnip vein-clearing virus (TVCV) | |
| 12 out of 16 samples produced a low-level positive response | |
| 2Confirmed experimentally at Agdia, Inc. | |
| 3Reported to detect Piper chlorosis virus (PChV), a possible novel Tobamovirus. | |
For a list of validated hosts, please see the Full Validation Report.
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Reagent Set for Ribgrass mosaic virus (RMV), 96 testwells
SRA 57601/0096
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Shown in cart
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Reagent Set for Ribgrass mosaic virus (RMV), 500 testwells
SRA 57601/0500
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Shown in cart
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Reagent Set for Ribgrass mosaic virus (RMV), 1000 testwells
SRA 57601/1000
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Shown in cart
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Reagent Set for Ribgrass mosaic virus (RMV), 5000 testwells
SRA 57601/5000
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Shown in cart
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