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PathoScreen® Kit for Rhizoctonia solani (Rhiz)

Rhizoctonia solani is a ubiquitous, soilborne fungal pathogen and an important limiting factor to global food production. This aggressive necrotroph exists as a species complex and causes serious disease on a diverse range of hosts, including row crops, ornamentals, vegetables, turfgrass and several species of woody plants. This ELISA test is a qualitative serological assay for the detection of Rhizoctonia solani (Rhiz).

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Rhiz ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • RUB6 enzyme conjugate diluent, 1X ready to use
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Isolate Names / Geographic Strain Locations Analytical Sensitivity
Rhizoctonia solani-11654 (Germany) (DSM 63002) 1:270 dilution of culture surface collection (pathogen titer unknown)
Rhizoctonia solani-12197 (Japan) (DSM 62716) 1:30 dilution of culture surface collection (pathogen titer unknown)
Rhizoctonia solani-2781-248F (AG-1) (DSM 843) 1:2430 dilution of culture surface collection (pathogen titer unknown)
Rhizoctonia solani-CBS 130.14 (AL, USA) (AG-4) (DSM 852) 1:810 dilution of culture surface collection (pathogen titer unknown)
Rhizoctonia solani-CBS 298.37 (AG-6) (DSM 903) 1:1,024 dilution of culture surface collection (pathogen titer unknown)
Rhizoctonia solani-R96 (Finland) (AG-5) (DSM 22880) 1:640 dilution of culture surface collection (pathogen titer unknown)
Rhizoctonia solani-R98 (Finland) (AG-3) (DSM 22847) 1:7290 dilution of culture surface collection (pathogen titer unknown)
Rhizoctonia solani-R114 (Finland) (AG-2-1) (DSM 22843) 1:2430 dilution of culture surface collection (pathogen titer unknown)

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit for Rhizoctonia solani (Rhiz), 96 testwells
PSA 41000/0096
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PathoScreen® Kit for Rhizoctonia solani (Rhiz), 288 testwells
PSA 41000/0288
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PathoScreen® Kit for Rhizoctonia solani (Rhiz), 480 testwells
PSA 41000/0480
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