PathoScreen® Kit for Prunus necrotic ringspot virus (PNRSV)

This ELISA test is a qualitative serological assay for the detection of Prunus necrotic ringspot virus (PNRSV), one of the causal agents of Rose Mosaic Disease, in prunus and rose leaves. PNRSV is a member of the Ilarvirus genus known for their non-enveloped, quasi-spherical-shaped virus particles.

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:1,350 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
PNRSV-A (ATCC® PV-295™) (MI, USA) PNRSV-CH3 (USA)
PNRSV-CH9 PNRSV-CH30
PNRSV-CH57 PNRSV-CH61
PNRSV-DPLP (ATCC® PV-34™) PNRSV-G (ATCC® PV-22™) (WI, USA)

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Prunus necrotic ringspot virus (PNRSV), 96 testwells
PSA 30500/0096
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PathoScreen® Kit, Prunus necrotic ringspot virus (PNRSV), 288 testwells
PSA 30500/0288
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PathoScreen® Kit, Prunus necrotic ringspot virus (PNRSV), 480 testwells
PSA 30500/0480
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